影像学对骨髓间质干细胞治疗脑卒中实验效果的评价.docVIP

影像学对骨髓间质干细胞治疗脑卒中实验效果的评价 马秀华，吕富荣,吕发金 ,黄铀新，肖智博 ,肖云华，杨柳 ，张瑜 (重庆医科大学附属第一医院放射科，重庆 400016) 摘 要：目的 探讨影像学检查在骨髓间质干细胞（mesenchymal stem cells, MSCs）治疗缺血性脑卒中实验研究中的应用价值。方法 雄性成年wistar大鼠，随机分假手术组（Sham组）、脑缺血组再灌注组（MCAO组），）、PBS治疗组和MSCs治疗组，每组分别于造模成功后1d，、3d，、7d,、 14d, 、28 d行CT灌注成像（CT perfusion imaging，CTPI）、MRI检查、神经功能评分、2,，3,，5-三苯基氯化四氮唑（2-3-5triphenylterazolium ，TTC）染色、HE染色及微血管密度分析。结果 ⒈ Sham组每时间点血流动力学未见明显异常，脑组织未见明显梗死区域。2. MCAO组、PBS组、MSCs组： = 1 \* GB3 ① CTPI参数图、T2WI图与TTC染色显示梗死体积差异无显著性染色显示梗死体积差异无统计学意义；MSCs组与MCAO组、PBS组比较，7 d、14 d、28 d梗死体积差异有显著统计学意义（性,Pp0.05）。 = 2 \* GB3 ② MSCs组与MCAO组、PBS组比较，rCBF在7 d、14 d、28 d升高，差异有显著性差异有统计学意义，（pP0.05）；神经功能评分减低明显，微血管密度明显增高,，HE染色示缺血周边区细胞变性坏死程度减低。⒊ MSCs移植治疗组3 d及7 d在梗死灶周围见点状长T1、短T2信号，14 d及28 d低信号向梗死病灶方向弥散，强度减低。结论 CTPI和MRI影像技术，可以从形态学及血流动力学方面观察MSCs治疗缺血性脑卒中的疗效，磁标记MSCs的MRI检查，实现了活体追踪标记细胞在脑内的存活、迁移及分化，为干细胞移植研究提供一个宽广的平台。 关键词：骨髓间充质干细胞；缺血性脑卒中；超顺磁性氧化铁颗粒；CT灌注成像；磁共振成像 Value of Iimaging to evaluate the experimental effects of Mmesenchymal stem cells therapy for ischemic stroke rats MA Xiu-hua, LVU Fu-rong, LUV Fa-jin , HuangUANG You-xin , XIAO Zhi-bo, XiaoIAO Yun -– h ua, YANG Liu, ZHANG Yu (Department of Radiology，, the First Affiliated Hospital, Chongqing Medical University of Medical Sciences ，, Chong qing 400016，, China) Abstract: Objective To investigate the value of imaging application forto evaluate the therapeutic effectexperiment of mMesenchymal stem cells (MSCs) transplantation in the treatment of ischemic stroke. Methods Totally 80 Aadult male Wistar rats were divided into sham operation group (n=5) , ischemic reperfusion (MCAO ) group (n=25), PBS treated group (n=25)and MSCs treated group (n=25),. MSCs were isolated from the bone marrow of femur and tibia of male Wistar rats at the age of 4 to 6 weeks and identified by flow cytometry followed by SPIO labeling. MCSs of 2×106 were injected into ischemic reperfusion rats through tail vein to MSCs treated group 24 h after reperfusion, and 1 ml PBS were given to PBS group at the same time point. and atIn 1d，3, 3d