异氟烷对老年大鼠海马胆碱乙酰转移酶的作用和空间学习记忆的影响.docVIP

编号：200906052 刊用形式： 文章编号：1000-5404(2009)24- 异氟烷对老年大鼠海马胆碱乙酰转移酶的作用和空间学习记忆的影响 罗铁山 ,陶国才 ,易斌 , 李鹏 ,石胜驰 （第三军医大学西南医院麻醉科，重庆 400038） 摘 要: 目的 探讨异氟烷对老年大鼠海马胆碱乙酰转移酶（Choline acetyltransferase，ChAT）表达的作用和空间学习记忆的影响。 方法 20月龄老年雄性SD大鼠54只，随机分为3组（n=18）。A组（对照组）：吸入30%氧气的空氧混合气体6h；B组（异氟烷单次吸入组）：单次吸入1.2%异氟烷和30%氧气的空氧混合气体6h；C组（异氟烷多次吸入组）：吸入1.2%异氟烷和30%氧气的空氧混合气体2h，1次/d，连续3d。各组处理中随机抽取5只行血气分析，5只完成处理24h后处死取脑分离海马，免疫组化法和RT-PCR法检测ChAT的表达，剩余24只行Morris 水迷宫实验检测大鼠的空间学习记忆功能，持续6d。 结 果 与A组比较，B、C组海马ChAT mRNA及蛋白表达下调（P0.05)；2d～5d的逃避潜伏期延长(P0.05）；学习记忆曲线后移，B、C组至第4d渐趋稳定，而A组第3d趋于稳定；停留原平台象限的时间和穿越原平台次数减少（P0.05）；B、C组差异无统计学意义（P0.05）。 结论 异氟烷可引起老年大鼠短期空间学习记忆功能减退,其机制可能与海马ChAT表达下调有关。 关键词： 异氟烷；胆碱乙酰转移酶；海马；空间学习记忆功能 中图法分类：R 971.2 文献标识码：A Effects of isoflurance on hippocampal ChAT expression of hippocampus and spatial learning and memory in aged rats LUO Teie-shan, TAO Guo-cai, YI Bin, LI Peng, SHI Sheng-chi (Department of Anesthesia, Southwest Hospital, the Third Military Medical University, Chongqing 400038, China) Abstract： Objective To explore the effect of isoflurance on the expression of Ccholine acetyltransferase（ (ChAT）) in the expression of hippocampus and spatial learning and memory function in aged rats. Methods A total of 54 20-month Sprague-Dawley male rats 54, were randomly divided into three3 groups with 18 rats each group.. Group A (the control group) received a breath of 30% oxygen and air mixed gas for 6 h; Group B (Single inhalation of isoflurane group) were anesthetized with 1.2% isoflurane、, 30% oxygen and air mixed gas for 6 h; Group C (Repeated inhalation of isoflurane group) were anesthetized with 1.2% isoflurane、, 30% oxygen and air mixed gas 2 h aevery day for 3 days. Five rats were randomly selected from each group for the arterial blood gas analysis in the course of treatment,. Another Five rats randomly selected from each group were killed and their hippocampus were immediately isolated for detection of ChAT expression by immunohist