益气滋肾合剂对牙周炎大鼠外周血TNF-α,IL-1β含量及牙槽骨微观形态的影响.docVIP

益气滋肾合剂对牙周炎大鼠外周血TNF-α、IL-β含量及牙槽骨微观形态的影响 杨 霞，向学熔，李丛华，黄孝庆 （400015重庆，重庆医科大学附属口腔医院口腔内科教研室） [摘要]目的　 观察益气滋肾合剂对牙周炎大鼠外周血TNF-α和IL-1β水平的影响，探讨其治疗牙周炎的机理。方法　选用80只牙体牙列完整，无龋坏及牙周病的5月龄Wistar大鼠，雌雄各半，体质量200～250g，随机分为5组：正常对照（n=10，N）组；模型对照（n=15，A）组，采用“牙颈部钢丝结扎+高糖饮食+激素”局部与全身因素相结合的方法，建立牙周炎动物模型；益气滋肾合剂（灌胃）高剂量[20 g/（kg·d），B1]、中剂量[10 g/（kg·d），B2]、低剂量[500 mg/（kg·d），B3]组（n=15）。12周后通过对大鼠一般状态、牙齿松动度、大鼠外周血中IL-1β、TNF-α含量的变化和大鼠牙槽骨微观形态分析，评价不同浓度的益气滋肾合剂治疗大鼠牙周炎的效果。结果 大鼠外周血中TNF-α、IL-βA组[（4.909±0.505），（0.318±0.086）]显著高于B1[（4.051±0.463），（0.253±0.193）]、B2[（4.085±0.374），（0.266±0.062）]组（P＜0.05）；Micro-CT成像分析得出骨小梁数量和骨小梁分离度值A组[（1.24±0.22），（789.3±157）]显著低于B1[（1.95±0.34），（362.3±62.7）]、B2[（1.88±0.39），（396.2±58.2）]组（P＜0.05）。结论 益气滋肾合剂可以降低大鼠外周血中IL-β、TNF-α的水平， [关键词]慢性牙周炎；肿瘤坏死因子-α；白细胞介素-1β，益气滋肾合剂 [中国分类号] R780.2 [文献标识码] A Effect of nourishing kidney tonifying qi mixture on levels of TNF-α and IL-1βin peripheral blood and pathological changes of experimental periodontitis rats Yang Xia， Xiang Xuerong， Li Conghua，Huang Xiaoqing.（Department of oral medicine，Stomatology hospital，Chongqing Medical University） [Abstract] Objectives To observe the effect of nourishing kidney tonifying qi mixture on the level of tumor necrosis factor-α（TNF-α）and cytokine interleukin-1β（IL-1β） in the peripheral blood during experimental periodontitis for it s treating mechanism. Methods Eighty 5-months-old Wistar rats were divided into five groups at random. Group N was killed at the beginning of the experiment and used as the normal control. GroupA，B1，B2，B3 were used as experimental periodontitis models. The rats in group A were killed at the end of experimental periodontitis establishment to assess the features of the model as the control groups. Group B1，B2，B3 received the therapy of the mixture with different dosage for 12 weeks and were killed respectively.The levels of TNF-α and IL-1β in the peripheral blood were measured with enzyme-linked immunosorbent assay after the rat s were killed.Results The levels of TNF-α and IL-1β in treated g