银花苦丁茶饮料对烫伤大鼠肾脏的保护作用.docVIP

银花苦丁茶饮料对烫伤大鼠肾脏的保护作用 柳 洁，肖聪颖，汪 冶，陈良富 （418000 怀化，怀化医学高等专科学校） ［摘要］ 目的 观察银花苦丁茶饮料对烫伤大鼠肾脏微细结构及血清TNFα与IL-1浓度的影响，评价其对肾脏的保护作用。方法 将50只雄性健康SD大鼠制成烫伤病理模型，用分层随机设计法将其均分成对照组与实验组。对照组饮生理盐水，实验组服银花苦丁茶饮料。按烫伤时间长短，分别将两组均等分成5小组。HE染色法检测肾实质的微细结构变化； SABC免疫组化与Mimics10.01图像分析法检测分析肾实质TNFα表达；ELISA检测血清TNFα与IL-1。 结果 烫伤后，两组大鼠肾实质均有不同程度的炎症与损伤性改变，血管球与肾小管上皮细胞有TNFα表达, 血清TNFα与IL-1升高。对照组肾实质炎症与损伤程度重，范围广，进展快，血管球充血与出血，肾小管上皮内可见各种变性细胞与裸核细胞， TNFα表达较实验组强，其灰度值与烫伤时间呈正相关（r1=0.99 P＜0.01），血清TNFα与IL-1浓度在烫伤后快速升高，烫伤后第2小时达高峰（89.81±5.93，63.25±4.29）。实验组肾实质的炎症与损伤程度轻，范围局限，进展缓慢，血管球充血而无出血，肾小管上皮内仅见颗粒变性细胞与轻度水样变性细胞，TNFα表达较对照组弱，其灰度值与烫伤时间呈负相关（r2=-1.00 P＜0.01），血清TNFα与IL-1浓度在烫伤后缓慢升高，烫伤后第4小时达高峰（77.61±15.20，47.84±2.78）。结论 银花苦丁茶饮料能延缓TNFα与IL-1的释放，对烫伤大鼠肾脏有一定的保护作用，可用作烧伤的辅助治疗。 ［关键词］ 金银花；苦丁茶；烫伤；肾；肿瘤坏死因子α；白细胞介素-1 ［中图法分类号］ ［文献标识码］ The Protective Effect of Honeysuckle Immature Chinese Holly Leaf Beverages to the Kidney of the Scalded Rats Liu Jie , Xiao Congying , Wang Ye , Chen Liangfu (Huaihua Medical Institute, Huaihua , Hunan Province, 418000, China ) [Abstract] objective to observe the influence of honeysuckle immature Chinese holly leaf beverages on the kidney microstructure and serum concentration of TNFα and IL-1, to estimate its protective effect on the kidney. Methods 50 male healthy SD rats made into the scalding pathology models and were divided HYPERLINK /dict_result.aspx?searchword=%e5%b9%b3%e5%9d%87tjType=sentencestyle=t=mean meanly with stratified randomized design method into the contrast group and experimental group, The former drank saline and the latter were given honeysuckle immature chinese holly leaf beverages. According to the length of scalding, the two groups were subdivided into five groups respectively. The microstructural changes of renal parenchyma were detected with HE, the TNFα expression were detected with immunohistochemical SABC and Mimics10.01 HYPERLINK /dict_result.aspx?searchword=%e5%9b%be%e5%83%8f%e5%88%86%e6%9e%90%e6%b3%95tjType=sentencestyle=t=image+analysis