胰岛素样生长因子-1在骨性Ⅲ类错合畸形中的表达.docVIP

胰岛素样生长因子-1在骨性Ⅲ类错合畸形中的表达 陈允嘉，李 艳，秘双燕，吴增波，王 强，王豫蓉 （400015重庆，重庆医科大学附属口腔医院上清寺院区正畸科） [摘要] 目的 研究在下颌骨发育过度导致的骨性Ⅲ类错合畸形患者中，胰岛素样生长因子-1（IGF-1）基因的表达，探讨IGF-1基因与下颌骨生长的关系。应补充相关性的分析。方法 选取骨性Ⅲ类错合畸形患者和骨性Ⅰ类患者（包括个别正常合）共54例各多少例，应用荧光定量PCR技术和酶联免疫吸附剂测定（ELISA）技术研究下颌骨中IGF-1基因的表达变化。结果补充回归系数 RT-PCR检测结果显示，骨性Ⅲ类错合畸形组中IGF-1 mRNA的表达量为（5.5410±2.0447）μg/μl，骨性Ⅰ类组中IGF-1 mRNA的表达量为（1.2821±0.2731）μg/μl，2组比较具有统计学差异（P＜0.05）。ELISA检测结果显示，骨性Ⅲ类错合畸形组中IGF-1蛋白的表达量为（84.1259±29.2947）μg/L，骨性Ⅰ类组中IGF-1蛋白的表达量为（22.4064±4.9312）μg/L，2组相比具有显著差异（P＜0.05）。结论 下颌骨生长量越大，IGF-1基因的表达量越高，表明IGF-1基因与下颌骨髁状突软骨生长成正相关关系。没有进行相关性分析，怎能得出这个结论？ 应补充相关性的分析。 各多少例 补充回归系数 没有进行相关性分析，怎能得出这个结论？ [关键词] 骨性Ⅲ类错合；胰岛素生长因子-1；下颌骨；遗传因素 [中图法分类号] [文献标志码] A [通信作者] 王豫蓉，E-mail： HYPERLINK mailto:rywmin66@ rywmin66@ Expression of insulin-like growth factors-1 in class Ⅲ malocclusion Chen Yunjia，Li Yan，Mi Shuangyan，Wu Zengbo，Wang Qiang，Wang Yurong (Department of Orthodontics，Hospital of Stomatoligy，Chongqing Medical University，Chongqing, 400015,China) Corresponding author：Wang Yurong，E-mail： HYPERLINK mailto:rywmin66@ rywmin66@ [Abstract] 对照中文修改Objective To approach the expression of IGF-1 between Class Ⅲ malocclusion and ClassⅠmalocclusion, and we will investigate the relationship between the expression of IGF-1 and the mandibular growth. Methods 54 patients of skeletal Class Ⅲ malocclusion and skeletal Class Ⅰ malocclusion were included, detect the expression of IGF-1 mRNA and IGF-1 protein of patients in Class Ⅲ malocclusion and ClassⅠmalocclusion by Real-Time polymerase chain reaction (PCR) and enzyme linked immunosorbent assay (ELISA) method . Results The detection result of RT-PCR method showed that the expression of IGF-1 mRNA in Class Ⅲ malocclusion group is (5.5410±2.0447)μg/μl ,which was significantly higher than that in ClassⅠ malocclusion group. ELISA assay showed the expression of IGF-1 protein in Class Ⅲ malocclusion group and ClassⅠmalocclusion group was (84.1259±29.2947)μg/L and (22.4064±4.9312)μg/L,respectively. There was signifi