细胞分选原理及其应用.pptVIP

panNK+ Selected from Mo Spleen Start NK+ Selected Data analysis 的技术要领 细胞稀释后，用吸管混匀细胞（不要倒置试管） 倒出未标记的细胞时，让液体连续流淌，试管停留在最后的位置约2秒钟，但不要刻意抖下最后一滴细胞液 * Hello every one. I am glad to be here and have this opportunity to give a talk about our StemCell Technologies company and our company’s products * Before we go into details of our products, let’s get it clear: what is cell separation and how to do it? Most samples such as blood and bone marrow are cell mixture. To study one type cell function, we need isolated the cell out of the mixture. This approach is cell separation. To separate cells, we usually label cells with antibody. Meanwhile, the antibody is labeled with fluorescence molecule or linked to a particle or some sort…, Then use fluorescence or particles to separate cells. * two basic approaches in cell separation, which are positive selection and negative selection. * Today I am going to focused on our sell separation productions. As you see here, we have several sell separation systems for you to choose. With these products, we can separate human cell, mouse, rat and rhesus monkey cells. * StemCell technologies has a unique way to separate cells. That is TAC technology. This graph show the TAC structure which is two mouse antibodies are cross linked by rat anti-mouse antibodies. In this case, one mouse antibody targets cell surface marker and the other binds to particle. This is called direct labeling. Another is called indirect labeling. That is cells are linked with labeled antibody, can be biotin or fluorescence labeled antibody and then use anti biotin or fluorescence TAC. Using the TAC technology, we developed our cell separation products. * By using our TAC technology, we developed following cell separation systems. RosetteSep is suitable for human whole blood sample It’s a negative selection Basic approach include cell labeling and density centrifugation Only need centrifuge * The mechanism behind this is we use two types of TAC. One target unwant