用套式PCR方法检出腹泻及健康犬粪中的犬冠状病毒3.PDFVIP

用套式 PCR 方法检出腹泻及健康犬粪中的犬冠状病毒 1 1 1 ,2 王玉燕 ,陆承平 ,温 　海 ( 1. 南京农业大学 农业部动物疫病诊断及免疫重点实验室 ,江苏南京 2 10095 ;2 . 公安部南京警犬研究所 ,江苏南京 2 100 12) Canine Coronavirus Detection in Feces f rom Diarrhea and Healthy Dogs by NestedPCR WAN G Yuyan1 , L U Chengping1 , WEN Hai1 ,2 ( 1. Key L ab of A ni m al d isease D i ag nos t ic an d I m m unol ogy M i nis t ry of A g ricul t ure , N anj i ng A g ric Uni v , N anj i ng 2 10095 , ) Chi na; 2 . N anj i ng Pol ice D ogs R esearch I ns t i t ute , N anj i ng 2 100 12 , Chi na Abstract : 112 f aecal samp le s of do gs collect ed f ro m A ugu st 2003 ～J anuary 2004 were t e st ed for t he p re sence of Cani ne coron av i r us (CCV) u sing a ne st edPCR wit h con served p rimer s for t he M gene . 18 out of 43 diar r hea f ece s f ro m do gs hou sed singly of N anj ing cit y were CCV po sitively , and t he CCV det ect able rat e were po sitively cor relat ed wit h sea son s , it wa s higher in wint er t han t hat of in summer and aut umn . 58 out of 69 f ece s f ro m heat hy do gs t rained in group s were po si tive for CCV , and t he p o sitive ratio of samp le s of do g school s f ro m Shenyan g (34/ 39) wa s higher t han t hat of f ro m N anj in g (24/ 30) . Sequence analy si s of 4 CCV M gene f ragment s , t wo f ro m po s itive diar r hea samp le s of N anj ing and t wo f ro m heat hy samp le s of Shenyang , showed t he p re sence of t he CCV s wit h high similarit y (94 . 8 %～96 . 7 %) to t