华中枸骨与守宫木快速繁殖的研究-园林植物与观赏园艺专业论文.docxVIP

摘要华中枸骨以带芽新梢茎段为外植体，研究了各种因素对该植物离体培养的影响， 摘要 华中枸骨以带芽新梢茎段为外植体，研究了各种因素对该植物离体培养的影响， 内容包括：外植体的采取、4C冷藏及消毒对植物初代培养的影响；基本培养基、生 长调节剂及培养方式对继代培养的影响；生根培养基及移栽、扦插基质的确定。结 果表明；外植体最好选取春季中上部萌条的新梢顶芽，4 0C冷藏则以22小时的处理 对消毒及后期生长比较有利，消毒方式以75％乙醇50秒、0．1％升汞6分钟、Tween．80 1滴为好；加琼脂的固态培养方式对继代培养较有利，对芽的诱导和增殖最佳的培 养基为 WPM+BA5mg／L+KT0．8mg／L+NA．AO．15rag／L+IBA0．1mg，L+GA30．2mg／L +O．2％活性炭+3％白糖+0．7％琼脂；生根培养的最佳培养基为1／2Nitch+IBA0．2mg／L +GA3 0．4 mg／L+O．2％活性炭+3％白糖+0．7％琼脂；生根苗移栽比较好的基质为珍珠 岩：泥炭土=3：7的配比；试管苗扦插比较好的基质为河沙：腐殖土=5：5的配比。 守宫木以带芽新梢茎段为外植体，研究了以下因素对该植物离体培养的影响： 初培中不同基本培养基对茎段培养的影响；继代中生长调节剂及培养方式对培养的 影响；生根培养基的确定。结果表明：B5的基本培养基对茎段的培养最有利；加琼 艏的固态培养方式对继代培养较有利：继代中对芽的诱导和增殖最佳的培养基为 B。+BA3mg／L+KT0．8mg／L+NAA0．25rag／L+0．2％活性炭+3％蔗糖+0．7％琼脂；生根培 养的最佳培养基为1／285+BAO．5mg／L+NAA0．2rag／L+GA3 0．8 mg／L+O．2％活性炭 +3％蔗糖+O．7％琼脂。 关键词：中华枸骨；守宫木；快速繁殖 AbstractDuring Abstract During the experiment of the rapid propagation of／／ex centrochinensis with shoot tips and lateral buds of new growth as explants，various factors have been studied， including explants’picking，refrigerating and sterilizing，the selection of basic medium， concentration of growth regulators，culture methods，the determination of roots growth medium，transplanting and cutting substratum．The results are as follows：explants which are picked—off in the superior part branchs in spring have better effects，and sprouting from shoot tips are more easier than from lateral buds；the refrigeration at temperature of 4。C for 22 hours is benefit for sterilizing and the sequent growth；the optimal sterilizing method is dipping into 75％ethanol for 50 seconds．then treating with O．1％corrosive sublimate and orle drop of Tween-80 for 6 minutes；culture in solid medium are benefitable in subculture；the optimal sprouting of buds and multiplication of adventitious buds is in WPM medium with the addition of BA5mg／L+KTO．8mg／L+NAA0．15m∥L +IBA0．1mg／L+GAs0．2mg／L+O．2％AC+3％sugar+O．7％agar；the better growth of roots is in 1／2Nitch medium with the addition of IBA0．2mg／L+GAs0．4 m∥L+O．2％AC+3％ sugar+0．7％agar；the optimal transpla