研究生分子生物学工作基础新基因研上究路线2.pptVIP

Immunoprecipitation Co-Immnunoprecipitation (co-IP) Co-IP Optimization Strategies Complex binding Use lysis and wash buffers with low ionic strength (i.e., 120mM NaCl) that contain non-ionic detergents (NP-40 and Triton X-100), which are less likely to disrupt protein-protein interactions. lysing cells by sonication or vortexing the lysate or bead-bound immune complexes during the wash steps should be avoided to prevent the disruption of the protein-protein interaction(s) of the target complex. Crosslinking the binding partners to strengthen protein-protein interactions. High background