人工感染鸭源新城疫病毒对鸭免疫功能作用的分子机制-畜牧学；动物营养与饲料科学专业论文.pdf.docxVIP

CONTENTSCoNTENTS CONTENTS CoNTENTS Abstract in Chinese ． ．．．I Abstract in English ．． ． ．． III 1 Introduction ． ．． ． ． ．1 1．1 ND ． ． ． ． ． ． ． ．．1 1．2 Innate immune system ． ． ． ． 2 1．3 Toll receptor—mediated signal transduction 3 1．3．1 Summary ofToll receptor ． 3 1．3．2 The identification ofToll receptor for the virus and bacteria ． ． ．．．．3 1．3．3 The Toll receptor mediated signal transduction ．．．． ． ．．． ． ．．4 1．4 RIG-I receptor—mediated signal transduction 5 1．4．1 The structure of RLRs 5 1．4．2 The identification ofRLRs receptor for the virus ． ． 5 1．4．3 RLRs receptor-mediated signal transduction 6 1．5 The introduction of host defense peptides and defensin ．．6 1．5．1 The introduction ofhost defense peptides ．． ．．． ． ．． ．．6 1．5．2The introduction of avian IB-defensins ．．7 1．6 The overview and classification ofcytokines 8 1．6．1 Interleukin ． ． ． ． ． ． ． ． ．9 1．6．2 Interferon ． ． ． ． ．． ．．． ． ．．．9 1．6．3 Tumor necrosis factor ． ． ． ．． ．． ．．． ．1 0 1．6．4 Chemokines ． ． ． ．．． ．．．． ．． ．．．． ．1 0 1．7 Purpose and significance ． ． ． ． ．． ．1 1 2 Materials and methods ．． ．．． ． ． ．12 2．1 Materials ． 12 2．1．I Experimental animals ． ．． ． ． ．． ．． ．．． ． ．12 2．1．2 virus ．．． ． ． ．．． ．．． ． ．．．． ．．．． ． ．．． ．12 2．1．3 Preparation of drugs and reagents ． ．．．．．．． ．．．．．． ．．．．．． ．．． 12 2．1．4 Instrumentation． ．．．．．．．．．．．． ．．．．． ．．． ．．．．．．．． ．．．．．．． ．．．．．．． ．．．． ． ． ．13 2．2 The detection ofantibody levels and morbidity mortality ．． ．．． ．．． ．．．13 2．2．1 Determination ofvirus virulence(EID50) 13 2．2．2 Experiment design and methods ．． ．． ．． ．． ．．．．． ．．．．． ．．．．．． ．．．．13 2．2．3 Detection of viral load for throat and cloacal swabs．． ．．．．．． ．．．．．．． ．．．．．．．．．． ．．．．14 2．2．4