灵芝三萜酸分批发酵的非结构动力学模型.PDFVIP

Mycosystema 菌 物 学 报 15 September 2011, 30(5): 767-773 jwxt@ ISSN1672-6472 CN11-5180Q ©2011 Institute of Microbiology, CAS, all rights reserved. 灵芝三萜酸分批发酵的非结构动力学模型 * 王晓玲 赵艳 刘高强 旷思敏 中南林业科技大学生命科学与技术学院 林业生物技术湖南省重点实验室 长沙 410004 摘 要：研究了灵芝胞内和胞外三萜酸在 30L 发酵罐中分批发酵的动力学特征。利用 Sigmoid 函数构建了灵芝细胞生长、底 物消耗、胞内和胞外三萜酸的非结构动力学模型，并根据 Boltzmann 拟合求解出各模型参数。结果表明，各模型预测值能够 较好地吻合实验实测值。灵芝细胞比生长速率在第 2.5 天达到最大值（μmax ），为 0.700d−1；葡萄糖比消耗速率在第 2.4 天达 −1 到最大值（qS, max ）为 1.060d ；胞内三萜酸比合成速率在第4.7 天达到最大值（qITA, max ）为 11.345mg/(g·d)；胞外三萜酸比 合成速率在第 5 天达到最大值（qETA, max ）为 10.077mg/(g·d)。灵芝胞内外三萜酸的合成和细胞生长均呈现部分偶联关系。 Sigmoid 模型在灵芝真菌生长和胞内外三萜酸形成规律的拟合方面有较好的适应性，研究结果为分批发酵法生产灵芝三萜酸 提供了理论依据。 关键词：药用真菌，灵芝，深层发酵，三萜酸，动力学模型 Unstructured kinetic models for triterpene acids of Ganoderma lucidum in batch fermentation * WANG Xiao-Ling ZHAO Yan LIU Gao-Qiang KUANG Si-Min Hunan Provincial Key Laboratory of Forestry Biotechnology, College of Life Science and Technology, Central South University of Forestry Technology, Changsha 410004, China Abstract: The dynamic behavior of intracellular triterpene acids (ITA) and extracellular triterpene acids (ETA) of Ganoderma lucidum by batch fermentation in a 30L stirred tank bioreactor was observed. Unstructured kinetics of cell growth, glucose comsumption, ITA and ETA were investigated based on Sigmoid model. The parameters of the model were determined by Boltzmann simulation, and the values predicted by the models matched well with those obtained from the observed. The maximum rate for specific cell growth was found to be 0.700d−1,