马氏珠母贝胆碱能免疫调节系统的初步研究-海洋生物学专业论文.docxVIP

优秀毕业论文 精品参考文献资料 明胆碱能系统还可以通过调节BMP7 明胆碱能系统还可以通过调节BMP7、Wnt和collagen参与调控机体的组织损伤 修复。 (4)乙酰胆碱受体基因的表达调控分析：基于miRNA与靶基因的调控机制， 本研究以课题组前期获得的miRNA转录组数据为基础，通过靶标分析软件 RNAhybrid和microTar预测获得miR一873、miR-669m、miR．4577、miR．103a、 miR．6753和miR．5 1 6b分别作为PmnAChR0【I、PmnAChRa2、PmnAChR0【3、 PmnAChRa4、PmnAChRa5和PmnAChR0【6的候选调控miRNAs；双荧光素酶报 告基因实验结果显示，miR．873使含有PmnAChRt]【l基因UTR的载体的荧光素 酶活性下调36．2％；miR．669m mimics对PmnAChRa2载体无影响；miR．4577使 PmnAChRa3载体的相对荧光素酶活性下调29．6％：miR．103a使PmnAChR0【4载 体的相对荧光素酶活性下调10．7％；miR一6753使PmnAChRa5载体的相对荧光素 酶活性下调27．3％；miR．516b使PmnAChRct6载体的相对荧光素酶活性下调 57．3％。本研究为进一步阐述胆碱能免疫调节系统在珍珠贝体内的免疫调控机制 奠定基础。 关键词： 马氏珠母贝，乙酰胆碱受体，LPS，免疫调节，real．time PCR Ⅱ 万方数据 Preliminary Preliminary Study on Cholinergic Immune Regulatory System in Pinctadafucata martensii Abstract The cholinergic immune regulatory system mediated by neurotransmitter acetylcholine play an important role in inhibiting the release of inflammatory cytokines which invovled in regulating the immune response of organisms．With previous research based the transcriptom database of Pinctada fucata martensii， expression of nicotinic acetylcholine receptors from haemocytes were significant increase after nucleus inserted surgery，therefore，indicating that cholinergic immune regulatory system was involve in the regulation of the immune response of pearl oyster after nucleus insertion．In this study，we selected the key genes of the cholinergic immune regulatory system through analyzing the genome and transcriptome database，and then these genes were cloned，the expression was detected in different tissues，their localization in the haemocytes was confirmed，and immune regulatory mechanism was analysed．The main results are follows： (1)Cloning and sequence analysis of nicotinic acetylcholine receptor genes：The six nicotinic acet)rlcholine receptor genes PmnAChRa 1，PmnAChRa2，PmnAChRa3， PmnAChRa4，PmnAChRa5 and PmnAChRa6 were cloned from Pinctada fucata martensii，which encoded for protein of 465，446，445，446，445 and 438 amino acids respectively．A