经筋微创疗法治疗类风湿关节炎临床疗效及对jakstat信号四通路的影响.docx

研究生优秀毕业论文 差异无统计学意义(D0．05)。两组治疗后滑膜组织中JAK—STAT信号通路信号抑制 差异无统计学意义(D0．05)。两组治疗后滑膜组织中JAK—STAT信号通路信号抑制 因子SOCSl蛋白表达较治疗前增加，差异有统计学意义(尺0．05)，但治疗组治疗后 较对照组治疗后下降更显著，差异有统计学意义(／KO．05)。(2)．滑膜组织病理结果 表明：经筋微创疗法可减轻滑膜细胞的增生及炎细胞浸润。 结论： 1．经筋微创疗法可改善RA患者的临床症状、实验室指标，缓解疾病活动度，疗 效颇佳。不仅能快速缓解关节局部疼痛，改善关节肿胀、僵硬及活动功能，而且能缩 短疗程，提高整体治疗效果。该疗法具有创伤小，不良反应少，弥补药物治疗不足的 特点，是中医外治疗法治疗活动期RA行之有效的方法，临床可进一步推广应用。 2．经筋微创疗法可有效降低关节滑膜组织中JAKl、p-JhKl、STAT3、p-STAT3、 TNF-a、IL-6的含量，提高SOCSl的水平，其作用机制可能是通过增加内源性GOCSl 的蛋白表达水平，降低JAK-STAT通路相关蛋白的表达，抑制JAK-STAT信号通路信号 传导，控制下游炎性因子的释放而发挥其抗炎作用。 关键词： 经筋微创疗法；类风湿关节炎；临床疗效：JAK-STAT信号通路蛋白；细胞 因子信号转导抑制因子1 Eva Eva I uat i on of the Therapeut i c Effect ofMer i d i an-S i new Re I ease therapyon Rheumatoid Arthr itiS and the anti—infIamatory mechan i Sill SpeciaI ity：Integrated Chinese and Western CI iniGal MediCine Author：Zhang Lu Tutor：Wei Song Abstract Objective 1．8y observing the clinieal manifestations and the change of inflammatory indexes in blood，to evaluate the efficacy of the Meridian—Sinew Release therapy for active rheumatoid arthri tis． 2．To observe the effects of Meridian-Sinew Release therapy for the expression and phosphorylation level of JAK—STAT signal transduction pathway signal protein：JAK、STAT3，signal transduction factor SOCSl，inflammatory factor TNF alpha，IL一6 and the pathologic morphotogy of synovial tissue， explore its Anti—inflammatory mechanism in treating rheumatoid arthritis． Method 1．clinical study：80 patients of RA met the diagnostiC criteria，inclusion criteria and exclusion criteria were randomly divided into treatment group and control group．The control group was treated with methotrexate(MTX)．The treatment group was treated with methotrexate(MTX)and Meridian—Sinew Release therapy，12 weeks of treatment，observation of the two groups before and after treatment in different time points of clinical manifestations and experimental index changes，record DAS28、ACR，evaluating its efficacy and safety．We use self—control and inter—group comparison． 2．Experimental study：Sel ect