向日葵染色体GiemsaC-分带研究-IngentaConnect.PDF

植物遗传资源学报2012,13(6):1078-1081 Journal of Plant Genetic Resources 向日葵染色体 Giemsa C-分带研究 1,2 2 2 高　 猛 ,安玉麟 ,孙瑞芬 1 2 ( 内蒙古农业大学农学院,呼和浩特010019; 内蒙古农牧业科学院生物技术中心,呼和浩特010031) 　 　 摘要:采用HKG(HCl-KOH-Giemsa)法对内葵杂3号三交种染色体进行了C-分带研究和分析。 结果表明:每条染色体至少 都有一条C-分带,染色体组共有62条C-分带,以中间带和着丝点带为主,中间带主要分布在染色体短臂上;C-分带强弱差异明 + + + + 显,其中46条强带,16条弱带。 Giemsa C-分带带型公式为:2n=2x=34=8I +3T +5I+I T +4C+2CI+4CI +3CI +I T + + + + + + CT +2CT 。 每条染色体都显示出显著的带纹特征,因此,利用Giemsa C-分带方法可以将向日葵的每条染色体区分开。 + 　 　 关键词:向日葵(Helianthus annus L.);染色体;Giemsa C-分带 Giemsa C-banding Patterns Analysis of Sunflower Chromosomes 1,2 2 2 GAO Meng ,AN Yu-lin ,SUN Rui-fen 1 ( Institute of Agronomy,InnerMongoliaAgriculture University,Huhhot010019; 2Biotechnology Research Center,InnerMongoliaAcademy of Agriculture andAnimal Husbandry Sciences,Huhhot010031) Abstract:In this paper,the HKG (Hcl-KOH-Giemsa)method studied and analysised the C-banding of chro- mosomes in triple cross of Inner Mongolia Hibrid oil sunflower3.The results showed that every chromosome had at least one C-band and there were 62 C-bands on the chromosome pairs,most of them were centromeric and inter- bands,and theinterbandsweremainly distributedontheshortarms;C-bandintensityweredifferent ,46werestrong + + + and 16wereweak.T