蛋黄卵磷脂对冻干红细胞恢复率的影响.PDFVIP

30 6 Vol.30,No.6 第 卷 第 期 蛋黄卵磷脂对冻干红细胞恢复率的影响 2009 2 年 月 December. 2009 0253-4339 2009 06-0049-04 文章编号： ( ) - DOI编码：10.3969/j.issn. 0253 4339. 2009. 06. 049 蛋黄卵磷脂对冻干红细胞恢复率的影响 周新丽 刘建峰 周国燕 张 今 刘宝林 (上海理工大学低温生物与食品冷冻技术研究所 上海 200093) 摘 要 蛋黄卵磷脂可以用来修复红细胞细胞膜在冻干过程中的损伤。将不同浓度、不同粒径的蛋黄卵磷脂加入红细胞样 品中，孵育不同的时间后冻干、复水，研究蛋黄卵磷脂浓度、粒径、孵育时间对冻干红细胞恢复率的影响。实验结果表 明，蛋黄卵磷脂能够提高冻干红细胞的恢复率，当蛋黄卵磷脂的粒径为0.8µm 、浓度为0.88mM 、孵育时间为15min 时，红 细胞恢复率达到(59.7 ±4.8)% 。 关键词 生物物理学；冷冻干燥；蛋黄卵磷脂；红细胞；恢复率 + 中图分类号：Q65; TQ028.6 3 文献标识码：A Effect of Egg Yolk Phosphatidylcholine on Recovery of Freeze-dried Red Blood Cells Zhou Xinli Liu Jianfeng Zhou Guoyan Zhang Jin Liu Baolin Institute of Cryobiology and Food Refrigeration, University of Shanghai for Science and Technology, 200093, ( Shanghai) Abstract Egg yolk phosphatidylcholine (EPC) can repair erythrocyte membrane damaged during the process of freeze-drying. The effects of EPC concentration, vesicle size and incubation time on the recovery of freeze-dried red blood cells were investigated in this study. The experimental results show that EPC would increase the recovery rate of freeze-dried red blood cells. When the EPC vesicle was 0.8µm, and the concentration was 0.88mM and the incubation time was 15min, the RBC recovery rate reached (59.7± 4.8)%. Keywords Biophysics; Freeze-drying; Egg yolk phosphatidylcholine EPC ; Red blood cells; Recovery rate ( ) 冷冻干燥法是保存红细胞的有效方法，具有 剂中加入磷脂，冻干复水后红细胞的溶血率降低到 常温保存、便于运输、保存费用低廉等优势