经前平颗粒对愤怒情绪反应大鼠模型γ氨基丁酸b2受体表达的影响及真核表达载体的构建中药学专业论文.docxVIP

优秀毕业论文 精品参考文献资料 目 目 录 引 言 ...................................................................................................................................1 第一部分 经前平颗粒对愤怒情绪反应大鼠模型 G A B A BR 2 表达的影响 3 实验一 经前平颗粒对愤怒情绪反应大鼠模型 GA BA BR 2 m RN A 表达的影响 3 材料与方法...........................................................................................................................3 1 材料 ...................................................................................................................................3 1.1 实验动物 ........................................................................................................................3 1.2 药品及试剂....................................................................................................................3 1.3 仪器及器材 ....................................................................................................................3 2 方法...................................................................................................................................4 2.1 大鼠模型制备 ................................................................................................................4 2.2 给药方法 ........................................................................................................................4 2.3 居住入侵实验操作........................................................................................................4 2.4 造模后行为学评价 ........................................................................................................4 2.5 大鼠脑组织总 R N A 的提取 5 2.6 RT-PC R 6 3 数据处理 ...........................................................................................................................8 结 果...................................................................................................................................9 1 大鼠造模后行为学评价 ...................................................................................................9 1