柿树炭疽菌突变体致病性及相关基因的克隆植物病理学专业论文.docxVIP

AbstractPersimmon Abstract Persimmon anthracnose is caused by Colletotrichum gloeosporioides Penz and occurs widely in many countries cultivating the persimmon all over the world．In recent years，Persimmon anthracnose also occurred seriously in the Chunan area, Zhej iang province and it became a limited factor for the development of persimmon industry．In order to learn the mechanisms of pathogen-host interaction，the pathogenesis··related mutants were obtained by Agrobacterium tumefaciens--mediated transformation(ATMT)and the genomic library of C gloeosporioides was constructed in our lab．On this basis，．the morphology and cytology of infection process of the mutants were investigated in this dissertation and the pathogenesis-related genes were cloned as well． 1 Morphology of C gloeosporioides mutants The culture and morphological characters of nine C gloeosporioides mutants (Cg一10、Cg-32、Cg一573、Cg一613、Cg-371、Cg一387、Cg-522、Cg一587 and Cg-605) were observed on PDA。plates．Meanwhile，the characters of colonies and conidial morphology of mutants were compared with that of the wild type contr01． The result of observation for characters of colonies showed that the mutants Cg一371，Cg-387，Cg一522 and Cg一613 were similar to w．ild type control，and other mutants were different with that．In addition，the account of mutant spotulation was less than that of the wild type，especially no conidia was observed in mutants Cg-1 0 and Cg一522 on their colonies． The result of morphological observation of conidia demonstrated that mutants Cg一32、Cg-522 and Cg一573 varied greatly in shape and some conidia of mutants Cg一32 and Cg-522 were ovoid，while mutants Cg一573 dumbbell—shape．Six mutants Cg-10、Cg一573、Cg-613、Cg-371、Cg-387、Cg·587 and Cg一605 were similarto the wild type in conidial morphology．The analysis of significant differences showed all conidial length of mutants were obviously different with that of the wild type，while conidial width were indifferent except mutants Cg一1 0、Cg一37 1、Cg一522 and