基于RNASeq绒山羊皮肤两种毛囊差异表达基因的分析.docVIP

基于RNA-Seq绒山羊皮肤两种毛囊差异 表达基因的分析 苏蕊乔贤范一星李晓凯张磊马宇浩王瑞军王 志英刘志红张燕军李金泉 内蒙古农业大学动物科学学院 自治区重点实验室 农业部肉羊遗传育种重点实验室 摘要： 为了研究绒山羊皮肤初级毛囊和次级毛囊之间的表达基因模式的差异，筛选影 响毛囊发育的重要基因，对6只内蒙古绒山羊阿尔巴斯型个体进行RNA-seq测序, 利用生物信息学方法对转录组数据进行分析，在测序结果质量控制后的基础上， 对差界表达基因进行筛选、功能注释和富集分析。共筛选到617个在初级毛囊和 次级毛囊中差异表达的基因，其中，上调表达基因297个，下调表达基因320 个。G0功能注释结果显示，上调表达基因主要行使电子传递链、呼吸链电子传 递、线粒体电子传递、基因表达等生物学功能；下调表达基因主要行使细胞吞噬、 蛋口质复性、中性粒细胞聚集、细菌真菌防御反应等生物学功能。利用KEGG数 据库作为参考，这些基因主要参与半胱氨酸和蛋氨酸代谢、RNA聚合、RNA可变 剪切、底物磷酸化等通路。PPP2R2A和CDK2AP1基因可能在皮肤毛囊发育中发挥 重要作用，以上结果为今后深入研究皮肤毛囊发育相关基因及功能提供了依据。 关键词： 绒山羊;毛囊;转录组;RNA-测序; 作者简介：苏蕊（1980-），女，内蒙古巴盟人，博士，副教授，主要从事绒山 羊遗传育种研究。E-mail: suruiyu@126. com 作者简介：李金泉（1957-），男，内蒙古土左人，教授，博士生导师，研究方 向：绒山羊遗传育种。E-mail: li jinquan nd@126. com 收稿日期：2017-07-03 基金：国家绒毛用羊现代农业产业技术体系项目（CARS-39-06） Analysis on Differentially Expressed Genes by RNA-Seq in Two Kinds of Hair Follicle in Cashmere Goats Skin SU Rui QIAO Xian FAN Yixing LI Xiaokai ZHANG Lei MA Yuhao WANG Ruijun WANG Zhiying LIU Zhihong ZHANG Yanjun LI Jinquan Inner Mongolia Key Laboratory of Animal Genetics, Breeding and Reproduction, College of Animal Science, Inner Mongolia Agricultural University; Abstract： This research is conducted to screen differentially expressed genes (DEGs) between the primciry and secondary hair follicle (I1F) of Cashmere goats skin to find candidate genes related to development. To analyze the transcriptome data by bioinformatics methods, the RNA-Seq of six individuals from Inner Mongolia Arbas Cashmere goat was performed. On the basis of quality control of the sequencing result, DEGs were idenlified as well as functional armotation and enrichment analysis was carried out. In this study, 617 DEGs has been selected from primary and secondary HF, and among them 297 are up-regulated while 320 are down-regulated. Additionally, the results of gene ontology (GO) expression elucidate that the main function of up-regulated genes are:electron transport chain, respiratory chain electron transport, mitochondrial electron transport, gene expression and other