ph敏感性羧甲基壳聚糖药物载体的设计制备及药物传递研究生物化学与分子生物学专业论文.docxVIP

摘要组织切片表明，叶酸修饰的靶向纳米粒子治疗组小鼠肿瘤组织的坏死最为严重。 摘要 组织切片表明，叶酸修饰的靶向纳米粒子治疗组小鼠肿瘤组织的坏死最为严重。 这些结果表明了靶向载药纳米微球相比于游离药物和普通载药纳米微球能更有 效的抑制肿瘤生长。 4)通过一种简单的方法合成了具有酸敏感性的原酸酯类交联剂(OEDE)， 在水溶液中使用交联剂交联羧甲基壳聚糖，制备了具有pH敏感性的水凝胶。 综上所述，本论文以羧甲基壳聚糖为材料设计的pH敏感性纳米粒子载体在 提高抗肿瘤效果的同时，降低了药物对正常组织的毒性，因此在肿瘤化疗领域具 有良好的应用前景。 关键词：羧甲基壳聚糖：药物传递：原酸酯：纳米粒子：水凝胶 IIl 万方数据 Chemotherapy Chemotherapy is of the common strategies for treatment of many types cancer in clinic．Unfortunately，most chemotherapeutic drugs have short half-life，low drug utilization rate，and can’t differentiate between normal and cells，and lead to severe side effects，therefore，the drug doses which can be controlled precisely to reach the tumor is one of the critical factors in chemotherapy，and drug delivery system is a fast developing way to achieve the above purpose．Drug delivery system refers to the different drug forms to get better therapeutic effect in the treatment of diseases．In this field，nano-drug delivery system and hydrogel drug delivery system are two common types．Nano-drug delivery system refers to the polymer material which have good biocompatibility were designed to load drugs with nanoscale particles to achieve a better therapeutic effect．When nanoparticles enter the blood circulation，nanoparticles can be passively target in tumor tissues to improve the concentration of drug in the tumor tissue and reduce the side effects of normal tissue because of the high permeability and retention effect in the tumor tissue effects(EPR) But the EPR effect only guarantee nanoparticles targeting around the blood vessels of tumor tissue and cannot effectively reach the tumor cells to limit the application of the drug．In order to solve this problem，The targeting groups which be integrated with receptors efficiently in the tumor cells were conjugated in the nanoparticles surface to prepare variety of carriers with active targeting function．For example，folic acid，transferrin，RGD peptides，galactose，benzene boric acid，etc Nano carriers which modified w