rna螺旋酶dhx32在肝细胞癌转移中的作用及机制研究临床检验诊断学专业论文.docxVIP

综上所述，我们的研究结果表明DHX32在肝细胞癌组织和高转移潜能肝癌 综上所述，我们的研究结果表明DHX32在肝细胞癌组织和高转移潜能肝癌 细胞株中表达上调，并与肿瘤血管侵犯和转移相关，高表达的DHX32能促进肝 癌细胞迁移和侵袭，此外，DHX32可能是通过促上皮．间质转化或通过与Racl 蛋白结合并上调其表达进而增强肝癌细胞的转移能力。因此，我们认为DHX32 在肝细胞癌中可能发挥促转移作用，其可能作为肝癌细胞获得更强转移潜能的生 物标志物，未来可能成为肝细胞癌抗转移策略新的治疗靶点。 关键词：DHX32肝细胞癌转移上皮．间质转化Racl Il 万方数据 AbstractHepatocellular Abstract Hepatocellular carcinoma(HCC)is one of the most lethal cancer globally．Many HCC patients had tumor recurrence after clinical therapy and the 5-year survival rate is under 30％，which is attributed to the tumor cell invasion and metastasis，and effective strategies in treating tumor metastasis sure to benefit patients．Hence，a growing body of research is focusing exploring the molecular mechanism of the development of HCC，and hope to exploit some key regulators to prevent target cancer metastasis． DHX32，a member of human RNA helicase，was ftrst to be reported of down—regulated in acute lymphoblastic leukemia and involved in lymphocyte differentiation．But，it has not been clarified of its biological function．Researchs from lab suggest that DHX32 was up—regulated in colorectal cancer and promoted CRC cell proliferation，migration，and invasion．However,the role of DHX32 in other solid tumors and the exact mechanism of its function has never been reported．Previous research of this study showed that DHX32 WaS over-expressed in HCC，which refers to another kind of malignant solid tumor,and its expression was aSsociated with tumor metastasis．Therefore，this study was conducted to investigate the role of DHX32 in the metastasis of hepatocellular carcinoma． First，we detected DHX32 mRNA and protein level in hepatocellular carcinoma tissues and their matched normal tissues by using real—time PCR assay and immunohistochemistry staining，meanwhile，we analyzed the correlation between its expression level and the clinical pathologic features of patients．Results displayed that DHX32 was up—regulated in HCC tissues compared with their adjacent nomal tissues and its expression was asso