乌蕨黄酮含量的季节动态成分分析及抗氧化抗肿瘤活性的研究植物学专业论文.docxVIP

分析结果表明它们都能诱导K562细胞凋亡甚至死亡，此外，几种典型细胞凋亡 分析结果表明它们都能诱导K562细胞凋亡甚至死亡，此外，几种典型细胞凋亡 特征经由荧光观察得到：膜气泡、核固缩、核碎裂、凋亡小体。 5．LC．MS成分分析 对乌蕨的各个部分和萃取相进行了LC．MS分析，经与己知化合物比对后， 结论如下：从乌蕨植物体中共分析出12种黄酮，地下部分所含的黄酮类化合物 相对较多。从乌蕨的5个萃取相中推测出13种黄酮，其中乙酸乙酯相和正丁醇 相所含的黄酮类化合物较多，这与5个萃取相黄酮含量的测定结果一致。 综上所述，乌蕨各部分的黄酮含量呈现明显的季节动态变化，冬季与春季 地上部分的含量较高，可以进行开发利用。同时，也发现了乌蕨的醇提物以及 有机萃取相都具有很好的抗氧化、抗肿瘤及抗酪氨酸酶活性，这可能与它所含 的某些黄酮类化合物有关。本文为乌蕨的药用开发提供了实验依据。 关键词：乌蕨；季节动态：抗氧化；抗肿瘤；抗酪氨酸酶 万方数据 AbstractStenoloma Abstract Stenoloma chusanum(L．)Ching is known as herbal healer．In folk，it shows efficacies in treatment．detoxification and hemostasis．S chusanum has been reported for its high flavonoid content，strong antioxidant potentials,antibacterial effects etc．However，the previous work still focuses its crude extract and there few data the biological ability and exact active ingredients in S chusanum．Hence， the purpose of this work is to evaluate the seasonal dynamics of the total flavonoids， chemical components and biological activities in s．chusanum．The present work will be of great significancence in the medicinal value and exploitation of S．chusanum The main results of this work are as followed 1．The seasonal dynamics of the total flavonoids and phenolic content and their antioxidant activity in S．chusanum The seasonal dynamics of the total flavonoids(TFC)and phenolic content(TPC) in different parts of S．chusanum and their antioxidant activity were investigated．The TFC and TPC in different parts ofS．chusanum obviously exhibited seasonal dynamic change．The content in the aerial part was higher than that in the subterranean part；The TFC and TPC in S chusanum relatively higher collected in winter and autumn，and in February the TFC and TPC was the highest，which was 24．63+1．34％and 9．58士0．4 1％．Additionally,the antioxidant activity including DPPH and ABTS free radical scavenging inhibitory,FRAP assay(reducing power)was investigated seasonally．The subterranean parts of S．chusanum manifested slightly higher antioxidant ability than the aerial parts；ac