粒细胞集落刺激因子对大鼠薄型子宫内膜治疗的初步研究妇产科学专业论文.docxVIP

优秀毕业论文 精品参考文献资料 硕 士 硕 士 学 位 论 文 英 文 摘 要 A B S T R A C T O B J E C T I V E T o investig ate th e effect an d p otential m ech an ism o f g ran u lo cyte colony -stim u latin g factor (G -C S F ) on th in en do m etriu m m od el in itially . M E T H O D 36 sex u ally m ature fem ale v irg in al S p rag ue D aw ley (SD ) rats w ith n orm al estrou s cy cles w ere selected , th in en dom etrium m o dels w ere estab lish ed by p erfusin g anh y drou s alcoh o l into uterin e cav ities for 5 m in utes, th en th ey w ere ran d om ly d iv id ed into fou r grou p s:(l)A cute end om etrial inju ry + G -C S F gro up ：G -C S F (4 0 fig/k g/d ) w as injected sub cu tan eou sly after th e m od el com p leted ev ery 2 4 h o urs for 5 d ay s. (2 ) A cute en dom etrial inju ry + contro l group : sam e do se p hy siolog ical salin e w as injected su b cutan eo u sly after th e m o del com p leted ev ery 2 4 h o u rs for 5 d ay s. (3 )C hron ic en dom etrial injury + G -C S F g rou p : G -C S F ( 4 0 |ag/kg /d ) w as injected sub cutan eou sly three estro us cy cles after th e m o d el com p leted ev ery 24 ho urs for 5 d ay s. (4 )C h ron ic en dom etrial m inju ry + control g roup ： sam e d ose phy sio log ical salin e w as injected sub cutan eo -u sly three estrou s cy cles after th e m o d el com p leted ev ery 2 4 h ou rs fo r 5 d ay s. A ll rats w ere k illed at th e fo urth estru s sin ce th e injection . T ak in g b ilateral uterin e tissu e for h isto lo gical ex am in ation , w e ob serv ed th eir end om etrialm orp h o lo gy ch an g es and m easured the end om etrialth ickn ess b y h em atoxy lin -eosin stain ing (H E ). A t th e sam e tim e w e detected th eir cytok eratin , v im entin exp ression p er un it en d om etrium area u sing im m u n oh istoch em istry techn ique. R E S U L T S il