tnfaip8家族在胃癌及心脏移植中的作用外科学专业论文.docxVIP

摘要裸鼠肿瘤质量与体积分别为(0．021+0．0039，178．13+26．95mm3)，组间差异显著， 摘要 裸鼠肿瘤质量与体积分别为(0．021+0．0039，178．13+26．95mm3)，组间差异显著， 具有统计学意义。 来氟米特显著的抑制移植后的急性排斥反应，减少炎性细胞的浸润，延长了 异种移植的生存期。TIPE2在心脏移植物中的表达随着排斥进展而升高，在来氟 米特组的表达低于3天组，差别具有统计学意义(P(0．05)。TIPE2在脾脏、淋 巴结的表达随着排斥进展而升高，在来氟米特组的表达高于于3天组，差别具有 统计学意义(P0．05) 结论：TIPE能够促进胃癌细胞的增殖，干扰TIPE的表达，可以增强胃癌细 胞的凋亡敏感性。提示TIPE可能参与了胃癌的发生、发展。TIPE可能为进一 步研究胃癌的发病机制提出一种新的思路。 TIPE2表达量随着免疫排斥程度不同而呈现不同变化。提示TIPE2可能参与 了免疫排斥反应，进一步探索TIPE2在移植免疫当中的可能机制，为寻找免疫抑 制新策略提供了曙光。 关键词：胃癌；TIPE；异种移植；TIPE2 万方数据 ABSTRACTABSTRACT ABSTRACT ABSTRACT Objective：As is known．TIPE and TIPE2 are important members of TNFAIP8 (Tumor necrosis factor-a induced protein-8，TIPE，TNFAIP8)family．Since TIPE is recently discovered antiapoptotic molecule，part of this study focuses constructing nude—mouse transplanted tumor model with the BGC823 cell strain--different expression of TIPE，and observing the influences TIPE has the growth of the transplanted tumor,in the of which expressions of TIPE in the clinical sample of gastric are collected and detected SO to analyze their relationship with clinical pathological characteristics of gastric cancer,as well to explore TIPE’S relationship with gastric cancer biological behavior and to figure out its effects the development of gastric TIPE2 is immune negative regulator,which plays an important part in balancing immune status．Another part of this study deals with building heart xenotransplantation model and collecting graft and spleen tissues at different time after transplantation to figure out the characteristics of TIPE2 protein and mRNA expression and the changes of cytokines， well to analyze their connections and to explore the possible functions of TIPE2 in the of xenotransplantation． Method：Part 1：Q．PCR method was used to detect and analyze TIPE mRNA expressions in 1 6 of gastric tissues and normal adjacent tissues．The results were combined with clinical pathological data to analyze the links between TIPE and gastric cancer occurrence and development． Part