超脉冲点阵co2激光辅助的药物传输对豚鼠黄褐斑模型治疗作用的研究外科学专业论文.docxVIP

Abstractobjective：Through Abstract objective：Through ultrapulsed fractional C02 laser establish drug delivery pathway treating melasma guinea pig model，testing model lesional melanocytes and melanin granules changes，explore ultrapulsed fractional C02 laser combined with tranexamic acid external application on melasma therapeutic significance spot． Method：30 healthy female guinea pigs，in which 20 of the model group，1 0 normal group．Cut off their back area hair about 4x4cm2． Model group were given daily intramuscular injection of 0．4％ progesterone，5ml／kg，while the 320nm wavelength ultraviolet irradiation the exposed back skin，once a day，60 minutes a time，for 3 0 days，guinea pig animal model chloasma．After modeling is complete，take the normal group and model group skin of guinea pigs，check the skin superoxide dismutase(SOD)and malondialdehyde(MDA)content,and histological observation，confirmed melasma guinea pig model successfully established．After modeling the skin of guinea pigs divided into four regions，namely：Group A：normal group；Group B：ultrapulsed fractional C02 laser treatment group；Group C：ultrapulsed fractional C02 laser+ tranexamic acid external application group；Group D：tranexamic acid external application group．Take normal skin of guinea pigs，model guinea pig skin，and 3 days，1 4 days，3 0 days after treatment guinea pig skin,HE staining，melanin granules staining(Masson Fontana staining)to observe changes in the number of melanin granules．Analysis of epidermal melanin granules number of changes before and after treatment using image analysis．The data entered into the computer with SPSS 20．0 software for statistical analysis to p0．05 Was considered statistically significant． III 万方数据 Results：20 Results：20 modeling guinea pigs，In the modeling process clearly visible stain formation，accompanied with skin redness，dryness，scaling and other reactions，20-30 days after irradiation showing a uniform and stable dark brown spots．Take after modeling skin of guine