二倍体和三倍体皱纹盘鲍繁殖期性腺发育生长与生化成份的比较研究水产养殖专业论文.docxVIP

二倍体和三倍体皱纹盘鲍繁殖期性腺发育、生长与生化成份的比较研究二倍体和三倍体皱纹盘鲍繁殖期性腺发育、生长 二倍体和三倍体皱纹盘鲍繁殖期性腺发育、生长与生化成份的比较研究 二倍体和三倍体皱纹盘鲍繁殖期性腺发育、生长 与生化成份的比较研究 摘 要 对繁殖期二倍体、三倍体皱纹盘鲍的性腺发育进行了组织学、超微结构和组织 化学观察，并对二者的生长和足肌的生化成份进行了比较研究。 结果表明：三倍体生殖腺发育受阻，大部分停留在休止期和增值期，雌性腺少数 发育到生长期和成熟期，产生少量的成熟卵母细胞，雄性腺只达生长期，没有精子形 成。超微结构观察，在卵母细胞发育过程中，二倍体卵母细胞细胞器发达，卵黄粒多 并均匀分布于细胞质中，三倍体卵母细胞细胞器较少，卵黄粒丰富。精子发生中，三 倍体溶酶体较多，其余细胞器少于二倍体，精巢中有形态畸形、滞育的次级精母细胞 和精子细胞。二倍体和三倍体组织化学观察结果基本相同。／养殖三年的三倍体壳长、 壳高、体重均明显小于二倍体，但繁殖季节其相对生长率高于二倍体，二者足肌的生 化成份含量无明显差异。)灿～ 关键词： 鲍}性‘腺发育，缒翌酉互二垂至商t～ 组织化学{《E韦§生化成份、 U J ’ 二倍体和三倍体皱纹盘鲍繁殖期性腺发育、生长与生化成份的比较研究Comparative 二倍体和三倍体皱纹盘鲍繁殖期性腺发育、生长与生化成份的比较研究 Comparative studies on gonad development，growth and biochemical composition in diploid and triploid abalones，Haliotis discus hannai during the reproductive period Abstract Gonad development in diploid and triploid abalones(Haliotis discus hannaO was observed by means of histological，ultrastructural and histochemical techniques during the reproductive period．Growth and biochemical composition of podium muscles were also studied． The results indicated that the gametogenesis in triploid abalones was retarded，the majority of which appeared to halt at the resting and proliferating stage．The minority of females developed into the growing and maturing stage wi也a few mature ova present． Males only developed into the growing stage and spermatozoa were not detected．During the period of vitellogenesis，odcytes of diploid abalones had developed organelles and numerous yolk granules which were evenly distributed in cytoplasm．In contrast,oocytes of triploids had less organelles but similar yolk granules．Triploid abalones possessed more lysosomes but less other organdies than diploids in the COUrSe of spermatogenesis．There were malformed or retarded secondary spermatocytes and spermatids observed in male reproductive system of triploids．We concluded almost the salTle results in terms of histochemical tests on diploid and triploid abalones．After three years of gro叭la，triploids were significantly smaller tha