繁茂膜海绵生物修复和生物监测近海养殖水体的可行性研究生物化工专业论文.docxVIP

AbstractAbstract Abstract Abstract Wantao Fu(Biochemical Engineerinl曲 Directed by Prof．Wei Zhang Diseases of aquaculture organisms and negative environmental impacts of pollutants in aquacultural water areas ale two key factors that restrain the increase in marine aquaculture production．As the oldest metazoan，sponges(Porifera) possess extreme abilities of pumping water to extract food and defense／immune mechanisms against a large number ofpathogens in seawater．Using all inter-tidal marine sponge Hymeniacidon perleve as a model system，this thesis aims to(1) investigate the characterigics of sponges as bioremediators to remove pathogenic bacteria,organic matters，and inorganic nutrients(N and P)；(2)clone且 perlevel4··3··3 mRNA as biomarker to biomonitor pathogenic bacteria in seawater． In the laboratory we demonstrate that the feasibility of using sponges as bio—remediator and bio-monitoring specie in integrated aquaculture ecosystem． To examine a biomarker from marine sponge H perleve in response to pathogenic bacteria in seawater,three partial 14-3-3 eDNA and partial 1 8s RNA ofH perleve were cloned and sequenced．The expression of 14-3—3 mRNA when exposed to non—infectious bacteria E coli was not significantly different from that ofcontr01．However,the expression of 14-3·3 mRNA when exposed to infectious bacteria矿anguillarum盯and矿alginolyticus was down—regulated respectively． The results demonstrated for the first time that sponges could differentiate the infectious bacteria from oon—infectious bacteria using the 14—3—3 mRNA transcript Ievel as a biomarkeL AbstractThe Abstract The tests of H perleve retaining and digesting pathogenic bacteria showed that盟perleve removed 96％ofE．coli with initial density 7．0—8．3×loo cells／mL during 1 0．5 h．and kept the pathogens growth under control at initial density 6．3× 10s cellstmL of 200 mL SNSW．E．coli and V anguilarum曩stained by fluorescence dye Dil were respectively fed to sponge．The laser confocal microscopy observation confirmed