反枝苋对噻吩磺隆的抗药性机制初探植物保护农药学专业论文.docxVIP

CONTENTSCONTEN CONTENTS CONTENTS Abstract in Chinese 。 ． ． ． I Abstract in English ．III 1 IntrOductiOn ．．1 1．1 The and harm characteristics of weeds 一1 1．2 The main control measures of weeds ．．1 1．2．1 Agricultural control ． ． ． l 1．2．2 Biological control ．．3 1．2．：；Chemical control ．3 1．3 The and development ofthe resistant weeds 4 1．4 The research overview of weed resistance ALS inhibitor 9 1．4．1 Characteristic of structure and function of the ALS and weed resistance ．9 1．4．2 The application ofALS inhibitors and resistant status 10 1．4．3 The resistance mechanisms ofALS inhibitors ．1 2 1．5 The purpose and the importance ofthis study． ． ． ．．14 2 Materials and methods ．．．16 2．1 Experimental materials ．．1 6 2．1．1 Experimental weeds 16 2．1．2 Spray equipment ．．16 2．1．3 Herbicide for experiment ．．16 2．1．4 Chemical reagent for study ．．16 2．1．5 Test instrument ．．17 2．2 Experiment method ． 。 ． 。。18 2．2．1 Method of resistant test of Amaranthus retroflexus to thifensulfuron-methyl ．．1 8 2．2．2 In vitro ALS activity assay ofAmaranthus retroflexus 19 2．2．3 ALS conservmive regions locus detection ofAmaranthus retroflexus ．．22 3 Results and analysis ． ． ．．25 3．1 The results ofresistance level test ofAmaranthus retroflexus to thifensulfuron-methyl ．．25 ：；．1．1 Petri．dish test ． ． ． ． ．．．25 ：；．1．2 Whole-plant test ． ． ．26 3．2 The rusults of in vitro ALS activity assay ofAmaranthus retroflexus ．．28 3．2．1 Protein standard CUrve 28 3．2．2 3-hydroxy ethyl ketone standard curve 28 3．2．3 The rusults of in vitro ALS activity assay ．29 3．3 The rusults ofALS conservmive regions locus detection ofAmaranthus retroflexus ．30 3．3．1 The rusults of DNA extraction and PCR amplification 30 ：；．：；．2 Sequence analysis results ．3 1 III 万方数据 东北农业大学农学硕士学位论文4 东北农业大学农学硕士学位论文 4 Discussion ．．33 4．1 Resistance level test ofAmaranthus retroflexus to thifensulfuron—methyl ．．33 4．2 The resistance mechanisms ofAmaranthus retroflexus to thifensulfuron-methyl