浮游动物对产毒微囊藻以及微囊藻产毒的影响地理学;环境地理学专业论文.docxVIP

；微囊II ；微囊 II AbstractAbstract Abstract Abstract It iS well known that one of the most harmful effects of cyanobacterial blooms iS the production of cyanotoxins．Recently the interactions between zooplankton and Microcystis species have been extensively concened．Some studies have revealed that zooplankton can cause the change of toxin production in Microcystis．In this paper the abundance of the potential microcystin-producing Microcystis(mcyB—containing Microcystis)in a small pool was monitored using the Real—time PCR,and the relationships between the potential mierocystin-producing Microcystis and zooplankton or nutrients were studied．Meanwhile，targeting on the transcriptions of genes responsible for microcystin synthesis under the stress of Daphnia，the response of Microcystis aeruginosa PCC7806 via the regulation of microcystin production toward the different stages ofDaphnia were studied using the RT-qPCR． ne results showed that the mcyB-containing Microcystis appeared in the studied area all the year and the concentrations of the mcyB-containing Microcystis ranged from 2．4 1 X 1 02 to 3．20 X 1 05 cells／m1．nle percentage of mcyB—containing Microcystis cells number in total Microcystis Was lower in winter and highest up to 39．73％in spring but went down to the lowest as 1．53％after the Microsystis bloom． It Was shown that the percentage of mcyB—containing Microcyst捃in total Microcystis had a positive linear relationship with the number of cladocerans，implying that the stress of cladoeerans Can induce the presence of toxic Microcystis in the bloom． The RT-qPCR Was firstly used to study the change of transcription regulation of genes responsible for microcystin synthesis in Microcystis aeruginosa PCC7806 under the stress of Daphnia．111e result showed that the grazing stress from D manga could increase transcript levels of mcyB,mcyD and mcyH as the inducible defence of 肱aeruginosa PCC7806．The change of microcystin content measured with ELISA agreed谢tll the change of tra