地锦草高效液相色谱指纹图谱研究有机化学专业论文.docxVIP

摘 摘 要 本文论述了中药色谱指纹图谱技术的概念，它在中药现代化进程中的意义和作 用，中药色谱指纹图谱方法特征，研究现状和发展趋势。本文还综述了地锦草近些 年的应用和研究情况，通过上述两方面论述明确了地锦草高效液相色谱指纹图谱研 究的科学意义和本项研究的创新性。 本论文通过对地锦草高效液相色谱指纹图谱的研究，优化了地锦草指纹图谱的 色谱条件，建立了地锦草药材的高效液相色谱指纹图谱，方法：用RP-HPLC法对不 同批次地锦草药材进行指纹色谱研究，色谱柱：CenturySILCl8-EPS5um250*4．6哪， 柱温为30℃，流速为0．8mL／min，检测波长为：O～lOmin：272nm，10～ 65min：345nm，以乙腈(A)和超蒸馏水(B)为流动相梯度洗脱。经过精密度、稳 定性、重现性等方法学考察，表明所建立的地锦草指纹图谱测定方法符合建立指纹 图谱的要求。地锦草的高效液相指纹图谱中地锦草主要有效成分均得到很好分离， 根据相似度分析处理结果，lO批次地锦草药材有15个共有峰，构成地锦草药材特 有的色谱指纹图谱特征，以没食子酸为参照物，对其它色谱峰的相对保留时间和相 对峰面积进行计算，作为技术参数评价指纹图谱，并利用Excel 2003软件对不同 产地和来源的地锦草药材指纹图谱进行了相似度比较，且相似度较好，为地锦草的 鉴别及品质控制提供依据。 关键词：地锦草；高效液相色谱：指纹图谱 Studying Studying of chromatographic fingerprint of Euphorbia humifusa Willd by HPLC Abstract This paper discusses the concept of chromatographic fmgerprinting technology，the significance in the process of Chinese herbal medicine modernization the characteristics of chromatographic fingerprinting method,the status quo and development fiends．This paper also reviewes the application and study of Euphorbia humifusa Willd in resent years, discusses the Euphorbia humifusa Willd HPLC fingerprint of the scientific significance and innovation． By studying on the Euphorbia humifusa Willd with High Performance Liquid Chromatography(HPLC)，found the best conditions and method to establish HPLC chromatogram fingerprint of Euphorbia humifusa Willd．Methods：The different places of the Euphorbia humifusa Willd ale measured by RP-HPLC．The separation is performed on Century SILCl8-EPS(靴m 4．6x250mm)column．The follow rate is O．8mL。min一，and the temperature is 30C．The gradient elution is in a mobile phase of acetonitrile(A)- superwater(B)at wavelength of 272nm(0-10rain)and 345nm(10-65rain)．The results suggest that it is satisfactory method because of good precision，stability，reproducibility， and other methods of investigation indicates that the fingerprints of the Euphorbia humifusa Willd is in line with the establishment of fingerprinting requirements．According to the similarity result，ten sa