食品中转基因大豆成分的定性和定量检测-中国食品卫生杂志.PDF

第一届 ICMSF —中国国际食品安全会议论文选登 食品中转基因大豆成分的定性和定量检测 赵卫东 　郑文杰 　刘 　　 　贺 　艳 (天津出入境检验检疫局 ,天津 　300201) 摘 　要 :为建立食品中转基因成分的定性定量分析 ,运用传统的定性 PCR 方法检测大豆加工产品 中转基因成分的存在 ,运用 Taqman 探针技术 ,对存在的转基因成分进行准确定量 。对于定量过程 Δ 中由于扩增效率的不同造成的误差 ,通过大豆的内源基因L ectin 进行校正 ,根据 Ct 值对应于校正 曲线计算出转基因大豆的含量 。本方法灵敏度达到 01 % ,误差范围小于 300 % 。可用于转基因 大豆的监测管理 。 关键词 :聚合酶链反应 ;植物 ,转基因;豆科 ;分子探针技术 Qualitative and quantitative detections of transgenic component in genetically modif ied soybean using realtime PCR ZHAO Weidong , ZHEN G Wenj ie , L IU Xuan , HE Yan ( Tianj in Entryexit Inspection and Quarantine Bureau of China , Tianj in 300201 , China) Abstract : Many countries have constituted higher requirement to the detection of GMproducts since a series of labeling regulations were put into practice . This requires accurate quantification of the transgenic material in the GMproducts. A conventional qualitative polymerase chain reaction ( PCR) assay was used to detect the presence of GMsoy and a realtime PCR was used to quantify the content of GMsoy . The errors due to differ ence in amplification efficiencies were amended by detecting the endogenous control . The percentage of geneti Δ cally modified soybean was calculated by Ct value through a standard curve . The sensitivity of the method could reach 01 % and the range of error was less than 300 %. Key Words : Polymerase ; Plants , Transgenic ; Legumes ; Molecular Probe Techniques 　　目前国际上还没有制定统一的定量检测的方法 聚乙烯吡咯烷酮40 、Tris 饱和酚 、氯仿 、异丙醇 、70 % 和标准[ 1 ,2 ] ,定量检测方法多是针对转基因作