生化分析仪试剂问交叉污染对总胆汁酸测定的影响及分析.docVIP

?论著?臨秦氏学嬪早妙。铲樽皿鬆3働 ?论著? 生化分析仪试剂间交叉污染对总胆汁酸测定的影响及分析 易向民，郑敏 （暨南大学医学院附属广州市红十字会医院，广东广州510220） 【摘要】 目的 探讨试剂间交叉污染对日立7600-010全自动生化分析仪演 ?，根据交叉 馬集顼副織眩翠痢繍奄氏眩綁林魁肴柯hl诚匣呛j懑感觸禪柚糖沛蛋门试剂对总胆汁酸测定有干扰影响「影悔。分析 总胆固礴St剂砒胆泮酸测國約予瘵報响采厳矽圄国唱瞩固斑锻M嗣旳汁酸的顺序，并車复十次.以确定总丿1H固总卿固醇对 总职汁截徇軫喻程此 細嘛呦懈m塔删柄丽擴常轴験潮弼购枷虬 确定总胆同醇试剂的「扰因素。丽I似删1删味 清洗程序，观察仪器特殊清洗程序排除试剂间干扰的效果。结果 与单独测定’ [对胆汁酸测 宛有爾剧ft豆桂?龟胭圜轄删J脚齡疲納前髒俯湖财湃M聊紘引起总胆汁酸测左结果明显升离的上滴帕删为 题斛稱畴K俩谢，御瘢I确懈翊劇婀補陥變贿能。纟繩飭卅册取曲御臟桝真荐疽盟爵的年懿， 牡洙塞铀竣數比诵潮弱南敢噸月并麻旌阀M陪缰舍奔餐臨：i嘩剛n舸删诉预料个硬爭用磺涵翩赦瞬清洗吸样 针，遽免您克畅I生。 【关霞词】 总胆固醇试剂；总胆汁酸；交叉污染 中图分类号：R446.1 文献标识码：A 宴章编号：：1674-4659 (2010) 03-0021-03 Influence of Biochemical Analyzer Cross-contamination between Reagents on Determination of Total Bile Adds YI XiangmuK ZHENG Min (Guangzhou Red Cross Hospital of Medical College of Jinan University, Guangzhou 510220, China) (Abstract] Objective To study the influence of the Total Cholesterol (TCHOL) reagents on the determination results of Total Bile Acids (TBA) by Hitachi 7600-010 automatic biochemical analyzer. Methods Mixed serum prepared, screened out the interference items of the determination of TBA according to the project of cross-contamination selection procedures; Defined the TCHOL which would have some influence on the determination of TBA. Determined three times of TBA after TCHOL measured, and repeated 10 times in order to detennine the impact of TCHOL on TBA? Low, medium and high concentrations of TBA were determined after TCHOL to define influence among the different concentrations of 1^A? Reagent 1 and 2 of TCHOL were added to mixed senim, to make sure which reagent would have cross-interference with TBA; Special cleaning procedures of equipment could exclude the effect of interference between the reagents. Results Compared with the group of determination of TBA independently, the reagents of TCHOL had a positive interference on The interference on low concentration of TBA was more obvious than that on the high one. Reagent 1 of TCHOL could significantly increase the determina? tion results o