蒺藜内生真菌发酵液抗菌及抗肿瘤活性初步研究.PDFVIP

微生物学通报 Dec. 20, 2013, 40(12): 22802287 Microbiology China © 2013 by Institute of Microbiology, CAS tongbao@ 研究报告 蒺藜内生真菌发酵液抗菌及抗肿瘤活性初步研究 武文斌 刘雅莉 岳高超 张雯* (华东师范大学 生命科学学院 上海 200241) 摘 要: 【目的】筛选出具有抗菌和抗肿瘤活性的蒺藜内生真菌。【方法】采用牛津杯法、 稻瘟菌模型及肿瘤细胞模型评价蒺藜内生真菌 PDB 和察氏培养基发酵产物的抗菌活性和肿 瘤细胞毒性。【结果】PDB 培养基发酵液和察氏培养基发酵液抑菌圈直径大于 10 mm 的菌株 分别占总菌株数的 19.05%和 23.81% 。PDB 培养基发酵液和察氏培养基发酵液对稻瘟菌的最 小抑制浓度(MIC)低于 10%的菌株分别占总菌株数的 19.05%和 47.61% 。对肿瘤细胞抑制率 高于50%的PDB 发酵产物占 PDB 发酵产物总数的 52.38%, 而对肿瘤细胞抑制率高于50%的 察氏发酵产物占察氏发酵产物总数的 28.57% 。【结论】部分蒺藜内生真菌的发酵产物具有抗 菌和抗肿瘤活性。 关键词: 蒺藜, 内生真菌, 抗菌, 抗肿瘤 Antagonism on microorganisms and cancer cells by the fermentation broth of endophytic fungi from Tribulus terrestris L. * WU Wen-Bin LIU Ya-Li YUE Gao-Chao ZHANG Wen (School of Life Science, East China Normal University, Shanghai 200241, China) Abstract: [Objective] The aim of the present study is to investigate the anti-microorganisms and anticancer activities of endophytic fungi isolated from Tribulus terrestris L.. [Methods] Endo- phytic fungi were cultured in PDB or Czapek medium to obtain different liquid fermentation. The 基金项目: 国家自然科学基金项目(No. *通讯作者: Tel: 86-21 : wzhang@ 收稿日期: 2013-02-19; 接受日期: 2013-06-08 武文斌等: 蒺藜内生真菌发酵液抗菌及抗肿瘤活性初步研究 2281 activities of fermentative products were tested by using Oxford plate assay system, Magnaporthe oryzae P-2b model and cancer cell model. [Results] The strains with their inhibition zone diameter of the PDB and Czapek broth greater than 10 mm were accounted for 19.05% and 23.