分子印迹电色谱整体柱研究有机化学专业论文.docx

中文摘要分子印迹是合成预定选择性固定相的新兴技术，电色谱整体柱是新型的高效微分 中文摘要 分子印迹是合成预定选择性固定相的新兴技术，电色谱整体柱是新型的高效微分 离技术，二者的结合集成了各自的特点，是当前的前沿课题。本论文主要工作如下： 1．首次合成了四种分子印迹毛细管整体柱。从整体柱的通透性，单体、交联剂和致 孔剂的选择和比例的调节上，系统研究了印迹整体柱的合成，通过电镜扫描和柱色谱 行为的测试，优化了柱制备条件，成功制备了以(R)一联二萘酚、对羟基苯甲酸、联 二萘胺和(S)．萘普生为模板的分子印迹整体柱。在电色谱上表现了良好的分子识别 能力和手性拆分能力，最高柱效可达70000 plates／m，显示了电色谱整体柱与分子印 迹结合所带来的高效率和高选择性。 2．系统研究了电色谱操作条件对分子印迹整体柱性能的影响。考察了电色谱条件， 如pH值、乙腈含量、盐浓度、电压及温度等因素，对分子印迹整体柱电渗流、保留、 柱效、分子识别和手性拆分的影响。在最佳电色谱条件下，印迹整体柱表现出良好的 分子识别和手性拆分能力。 3．对印迹整体柱色谱峰的不对称性进行了初步研究。考察了进样量和整体柱流速对 印迹分子峰不对称性因子影响，表明采用原位聚合的方法制各分子印迹聚合物，结合 整体柱和电色谱高效率的特点，是改善分子印迹峰不对称性的可行途径之一。 4．研究了电色谱分子印迹整体柱的柱效理论和印迹分子在电色谱模式下的保图机理。 通过对印迹分子和硫脲在印迹整体柱和空白柱上板高与流速的关系研究，表明分子印 迹整体柱由于高含量交联剂的存在，导致van Deemter方程中高的c项，并得出印迹 整体柱不宜于高速分离的结论。 关键词：分子印迹毛细管电色谱整体柱(尺)．联二萘酚对羟基苯甲酸联二萘胺 (S)．萘普生 AbstractMolecular Abstract Molecular imprinting is a new approach to prepare predictable selectivity stationary．Capillary electrochromatographic(CEQ monolith is a novel high performance micro·separation technology．The combination ofthese two technologies comprise their merits and has been the promising field．In this paper．research works on these were done as the following． Four novel molecularly imprinted polymer(MIP)capillary monolith were developed．The polymerization condition on the column permeatration and chrial separation WaS studied，including the choice and composition offunctional monomers，CROSS·linker and porogens．With the electron scanning micrograms and CEC，optimum polymerization condition was obtained and preparation of monolithic molecularly imprinted polymer of㈤一1，1一binaphthol，4-hydroxybenzoic acid，1，1-binaphthyl -2，2-diamine and(S)一naproxen Was successfully done．Good molecular recognition and enantiomer separation was achieved．The largest column efficency was 70000 plates／m．Our research shows high efficiency and high selectivity derived from the combination of CEC monolith and molecular imprinting Effect of CEC parameters on MIP monolithic column Was studied systemically，which including pH value，content ofacet