乳酸乳球菌胞外多糖的分离及其体外抗氧化活性研究.PDF

232 2009, Vol. 30, No. 01 食品科学 ※生物工程 1 1,2, 梅秀明 ，潘道东 * (1.南京师范大学国家乳品加工技术研发分中心，江苏 南京 210097 ；2. 宁波大学生命科学与生物工程学院， 浙江宁波 315211) ：本实验对乳酸乳球菌胞外多糖(EPS) 的离心除菌、提取、粗胞外多糖中蛋白质的去除进行研究，同时探 究胞外多糖的体外抗氧化活性。结果表明：6000r/min 离心15min；温度15℃；旋转蒸发浓缩到原体积的1/4；醇 沉最佳条件为３倍糖溶液体积的90% 乙醇沉淀12h；选择TCA 沉淀蛋白，TCA 的最佳浓度为10%；胞外多糖有显 著的体外抗氧化性。 ：乳酸乳球菌；胞外多糖；分离提取；抗氧化性 Study on Isolation and Antioxidant Activity in vitro of Exopolysaccharide Produced by Lactococcus lactis subsp. lactis 1 1,2, MEI Xiu-ming ，PAN Dao-dong * (1.Branch of Nationol Dairy Processing Technology Developing Center, Nanjing Univesity, Nanjing 210097, China ； 2.College of Life Science and Biotechnology, Ningbo University, Ningbo 315211, China) 　 Abstract ：Centrifugation conditions for isolate exopolysaccharide produced by Lactococcus lactis subsp. lactis, concentration multiple of exopolysaccharide solutiom via rotary evaporation, alcohol precipitation conditions and protein removal nethod were studied, as well as the antioxidant activity of the obtained exopolysaccharide by the above procedures. The results indicated that the yield of the exopolysaccharide can reach the highest under the centrifugation conditions of speed 6000 r/min, time 15 min and temperature 15 ℃, concentration multiple 4 times, alcohol precipitation conditions of 90% alcohol volume 3 times as much as exopolysaccharide solutiom and precipitation time 12 h, and concentration of TCA for removing protein 10%. The exopolysaccharide has distinct antioxidant ability in vitro. Key words ：Lacto