磁性高分子微球分离抗体的研究生物化工专业论文.docxVIP

摘要本文旨在合成一种可以快速、经济分离蛋白质的磁性高分子微球。以聚乙烯 摘要 本文旨在合成一种可以快速、经济分离蛋白质的磁性高分子微球。以聚乙烯 吡咯烷酮(PVP K-30)为分散剂，甲基丙烯酸缩水甘油酯(GMA)为聚合单体， 三(2一甲基丙烯酸甲酯)丙烷(刚)为交联剂，在水与乙醇体系中，利用偶氮 二异丁腈．(A珥N)引发分散聚合反应，合成了刚性、单分散的高分子微球，简 称PGMA．TRI；然后将合成的微球用无水亚硫酸纳处理修饰上磺酸基，制成阳离 子交换介质；最后利用离子交换和扩散作用将Fe2+导入微球，并应用渗透一沉积 法将纳米Fe304沉积在微球内部，得到具有刚性、单分散、超顺磁性的高分子微 球，简称M．PGMA．nU。其主要创新在于在该制备方法引入了交联剂，且加入量 可达单体质量的2％。利用粒度分析仪、环境扫描电子显微镜、震动样品磁强计 等仪器对微球的形貌、粒径分布、磁性能等进行表征。结果显示制备的微球平均 粒径5岫1左右并呈单分散性；微球形态完整表面光滑无孔；具有超顺磁性，比 饱和磁化强度为13．001 emu／g，在永磁铁作用下可以快速从液体里分离出来。并 且同时与不加交联剂的磁性微球M-PGMA进行了各种性能的对比。 利用免疫球蛋白(IgG)作为模型蛋白描述制备介质的蛋白吸附特性，适宜 条件下，微球的静态吸附容量可以达到287．2mg／g，并且在反复的吸附、洗脱、 回收实验中吸附容量和磁特性一直保持稳定。对微球的化学稳定性实验显示微球 在某些酸、金属离子、氧化剂中性质稳定。以上结果显示出了这种阳离子交换介 质在实际蛋白吸附、分离应用中具有很大应用潜力。 关键词：分散聚合法；渗透一沉积法；单分散；磁性高分子微球；蛋白吸附； ABSTRACTThis ABSTRACT This article describes the fabrication of a rigid magnetic monodisperse bead (M—PGMA-TRI，4．92㈣with polyglycidyl methacrylate(PGMA)cross-linked by trimethylolpropane trimethacrylate(TPd)．This was realized by adding a proper amount(2％w／w)of TRI after 3 h of the dispersion-polymerization reaction with the monomer of GMA．The mono—sized microspheres were fulthelprocessed to introduce magnetic granules by sulfonation and penetration-deposition approaches．The monodisperse bead(M-PGMA)without TRI addition was also fabricated for comparison．The morphology,size and magnetic characteristics of the microspheres weIe extensively characterized．The M-PGMA-TRI microspheres were nonporous，of smooth surface and superparamagnetic with a saturation magnetization of 1 3．0 emu／g． Recycled use of the material for protein adsorption exhibited stability of the magnetic properties of the M—PGMA-TRI，as compared to the significant loss of the saturation magnetization of the M—PGMA．The chemical stability of the M-PGMA—TRl was also confirmed bY examining its protein adsorption and magnetic properties after incubation in various solutions such as acidic buffer(pF2．2)for 24 h．The adsorption capacity of?-globulin reached 287．2mg／g and kept stable in the repeate