交变磁场对内蒙古河套蜜瓜生理生化特性的影响-微生物学专业论文.docxVIP

内蒙 内蒙古科技大学硕士学位论文 field with treating for 15 min resulted in reduced respiration rates, lower electrolyte leakage, delayed softening and decomposition, slowed the flow of titratable acid, and reduced flows of soluble solids. The results showed that the magnetic field with treating for 15 min was the best for maintaining good quality of Cucumis melo L. cv Hetao. (3) Thirdly, the quantitative PCR technique was used to detect the change of expression of PLD gene in Cucumis melo L. cv Hetao, which was treated by the different time of the alternating magnetic field. The results showed that when the treating time was 15min, the expression of PLD gene was the lowest. Under this condition, the Cucumis melo L. cv Hetao could maitain a good quality. To sum up, application of the alternating magnetic field prior to storage, especially when the treating intensity of 180V, treating time for 15min, showed beneficial effects on reducing negative responses and delaying ripening in Cucumis melo L. cv Hetao. Key Words: Alternating magnetic field; Cucumis melo L. cv Hetao; Physiology and biochemical characteristics; Phospholipase D (PLD); Quantitative PCR technique IV 目 录 摘 要 I ABSTRACT III 引 言 1 1 文献综述 2 1.1 河套蜜瓜 2 1.1.1 河套蜜瓜概述 2 1.1.2 河套蜜瓜的采后生理机制 2 1.2 磁场的生物效应 3 1.2.1 磁场概述 3 1.2.2 生物磁现象及生物磁效应 3 1.2.3 生物电磁学的应用现状 4 1.3 果蔬的贮藏保鲜 5 1.3.1 热处理保鲜技术 5 1.3.2 气调贮藏保鲜技术 5 1.3.3 涂膜保鲜技术 6 1.3.4 臭氧贮藏保鲜技术 7 1.3.5 河套蜜瓜的贮藏保鲜方法 7 1.4 磁场处理在果蔬贮藏保鲜中的应用 9 1.4.1 磁场处理对凤尾菇生长效应的影响 9 1.4.2 磁场处理对草莓保鲜效果的影响 9 1.4.3 交变磁场处理对莲藕切片保鲜效果的影响 9 1.4.4 交变磁场对葡萄贮藏保鲜效果的影响 10 1.5 磷脂酶 D 10 1.5.1 磷脂酶 D 概况 10 1.5.2 PLD 在抗逆反应中的作用 11 1.5.3 PLD 基因全长的克隆技术 12 1.6 选题目的 13 1.7 研究内容 13 2 不同强度磁场处理对河套蜜瓜采后生理特性的影响 15 2.1 实验技术路线 15 2.2 实验材料与方法 15 2.2.1 实验材料准备 15 2.2.2 实验方法 18 2.2.3 数据处理 21 2.2.4 结果与分析 21 2.3 小结 26 3 不同时间磁场处理对河套蜜瓜采后生理特性的影响 27 3.1 实验技术路线 27 3.2 实验材料与方法 27 3.2.1 实验材料准备 27 3.2.2 实验方法 27 3.2.3 实验数据处理 28 3.2.4 结果与分析 29 3.3 小结 32 4 不同时间磁场处理对鲜切河套蜜瓜 PLD 基因表达量的影响 33 4.1 实验路线图 33 4.2 河套蜜瓜 PLD 引物的确定 33 4.2.1 实验材料与仪器 33 4.2.2