电针对帕金森病模型大鼠内质网应激IRE1α-ASK中国康复医学杂志.PDF

ChineseJournalofRehabilitationMedicine, Jun. 2018,Vol. 33, No.6 ·基础研究· 电针对帕金森病模型大鼠内质网应激 IRE1α-ASK1-JNK通路的影响* 1 1 1,2 1 1 1 马 骏 王中明 王述菊 余沛豪 王 彬 王 琪 摘要 目的：探讨内质网应激介导的IRE 1α-ASK1-JNK 通路在鱼藤酮诱导的帕金森病（PD ）模型大鼠中的作用及电针干预 治疗对该通路的影响。 方法：将120 只健康雄性SD 大鼠随机分为正常组、假手术组、模型组、电针预治疗组、电针治疗7d 组、电针治疗14d 组、电针治疗21d 组、电针治疗28d 组，每组15 只，采用颈背部皮下注射鱼藤酮法制备帕金森病模型，假手术组只注 射不含鱼藤酮的等量葵花油乳化液。正常组、模型组、假手术组不做任何治疗；电针治疗组在造模完成后选取“风 府”、“太冲”穴给予电针治疗；电针预治疗组电针治疗7d 后再造模。采用旷场试验检测大鼠行为学改变，并用West- ern-blot 法检测大鼠中脑黑质内IRE 1α、ASK 1、p-JNK 蛋白表达的变化。 结果：模型组大鼠表现出明显的PD 症候群特征，电针干预后模型大鼠行为学评分改善明显（P ＜0.01 ）。模型组大鼠 黑质IRE 1α、ASK 1、p-JNK 蛋白表达较正常组和假手术组显著增高（均P ＜0.01 ）；与模型组相比，电针预治疗组和电 针治疗7d、14d、21d、28d 组IRE 1α、ASK 1、p-JNK 蛋白表达水平显著降低（均P ＜0.01 ）。 结论：电针防治帕金森病的机制可能与电针能明显抑制内质网应激通路IRE 1α-ASK1-JNK 的活性，保护多巴胺能神 经元免于凋亡有关。 关键词 帕金森病；电针；内质网应激；跨膜蛋白激酶1；凋亡信号调节激酶1；C-Jun 氨基酸末端激酶 中图分类号：R742.5, R245 文献标识码：A 文章编号：1001-1242（2018 ）-06-0658-05 Effects of electroacupuncture on the IRE1α-ASK1-JNK pathway of endoplasmic reticulum stress in rats with Parkinsons disease/MA Jun, WANG Zhongming, WANG Shuju, et al.//Chinese Journal of Rehabilita⁃ tionMedicine, 2018, 33(6): 658—662 Abstract Objective: To explore the action mechanism of the IRE 1α-ASK1-JNK pathway mediated by endoplasmic reticu- lum stress (ERS) in rats with Parkinson ’s disease induced by rotenone and the effects of electroacupuncture on it. Method: Male SD rats were randomly divided into normal group, sham-operation group, model group, pretreat- ment group, four EA groups (treatment for 7 days, 14 days, 21 days, 28 days), 15 rats in each group. PD model was induced by subcutaneous injection of rotenone at the rat ’s nape, and rats in the sham- operation group were injected with sun-flower oil that did not include rotenone. Rats in the EA groups were treated by EA at “Fen