医学毕业慢性活动性乙型肝炎外周血t细胞亚群上活化抗原的表达.docxVIP

XX大学 毕业论文 慢性活动性乙型肝炎外周血T细胞亚群上活化抗原的表达 2014年6月25日 慢性活动性乙型肝炎外周血T细胞亚群上活化抗原的表达 关键词：慢性;乙型肝炎;T淋巴细胞;CD69;HLA-DR 摘要:目的探讨慢性活动性乙型肝炎(CAHB)患者外周血活化抗原CD69、 HLA-DR分子在T细胞表达的变化及其意义。方法 以31例正常人作为对照， 采用免疫荧光三色标记流式细胞术，检测30例CAHB患者外周血T细胞亚群及 其CD69、HLA-DR抗原表达;同时用荧光定量PCR检测患者的病毒DNA载量。 结果CAHB患者外周血CD3+T细胞的百分率(63.78%±9.70%)与止常对照组 比较差异有显著性(P0.01), CD4 + T细胞的百分率(45.88%±8.51%)明显低于 正常对照组(P0.001), CD8 + T细胞的百分率(44.64%±7.10%)明显高于正常 对照组(P0.001) ;CD3 + T细胞CD69的表达(6.85%±2.83%)与正常人对照组 比较差异也有显著性(P0.01),其中CD4 + T细胞CD69的表达(2.31%±0.94%) 与正常对照组比较差界无显著(P0.05), CD8 + T细胞表达CD69水平 (3.87%±1.81%)明显高于对照组(P0.01) ;CD3 + T细胞上HLA?DR的表达 (17.09%±4.23%)与正常对照组之间差异有显著性(P0.001),其中CD4 + T细 胞中HLA?DR +细胞(7.97%±2.43%)和CD8 + T细胞中HLA-DR +细胞 (5?52%±1?48%)均比正常对照组显著增高(P0.01) (P0.001) ;CD8 + CD69+、 CD8 + HLA-DR +细胞的数量与病毒DNA载量之间呈正相关。结论CAHB患 者存在细胞免疫功能紊乱和淋巴细胞异常激活现象,T淋巴细胞的激活以CD8+T 细胞为主，其在抗病毒感染屮起着重要作用。 关键词:慢性;乙型肝炎;T淋巴细胞;CD69;HLA-DR The expression of activated antigens in peripheral blood T lymphocyte subsets in patients with chronic active hepatitis B. Abstract:Objective To investigate the expression of special marker for activation of peripheral blood T lymphocytes in patients with chronic hepatitis B virus and 让s significance. Methods Immunofluorescent three-color flow cytometry was used to study the expression of CD69and HLA-DR on T lymphocytes in30paticnts with chronic active hepatitis B virus and31 normal controls.The viral load was determined by fluorescence quantitative PCR? Results Expression of CD3 + T cells were significantly lower in pa-tients (63.78%±9?70%) than that in controls (P0.01), meanwhile the expression of CD4 + T cells was45.88%±8.51%, significantly lower than that of the controls ( P0.001 ) and the expression of CDS + T cell was44.64%±7.10%, significantly higher than that of the controls (P0.001) .Detection of CD69on CD3 + T cells in patients was6.85%±2.83%, significantly higherthan that of the controls (PO.01) .NO significant difference in the expression of CD69on CD4 + T cells was observed be-tween patients and the con