电化学发光免疫法与放射免疫法测定血清铁蛋白的评价.docVIP

电化学发光免疫法与放射免疫法测定血清铁蛋白的评价 电化学发光免疫法与放射免疫法测定血清铁蛋白的评价 作者:李桂彩，陈德平，肖静坤 【关键词】 电化学发光免疫分析法; 放射免疫分析法;血清铁蛋白 ,摘 要, 目的:评价电化学发光 免疫分析法(ECLIA)和放射免疫分析法(RIA)在测定血清铁蛋白(SF) 中的应用。方法:用ECLIA、RIA分别对中浓度SF的标本进行回收试 验和抗干扰试验(干扰物为血红蛋白)，对低、高浓度SF的标本进行 重复试验。结果:平均回收率ECLIA为95.6%、RIA为93.5%，两种 方法比较，差异无显着性(P0.05);变异系数(CV) ECLIA为3.0%、4.3%， RIA为8.5%、9.3%，两种方法比较，差异均有显着性(P0.05);抗血 红蛋白(Hb)干扰能力，当血清中Hb浓度在1.0 g/L,6.0 g/L时，随 Hb含量的增加，两种方法测定SF时的干扰都逐渐增加(正干扰)，血 清中Hb浓度?4.0 g/L用ECLIA测定SF和血清中Hb浓度2.0 g/L 用RIA测定SF时，其结果的偏差系数(CB)都5.0%。结论:两种方法 测定SF准确性、精密度均符合要求，且ECLIA优于RIA;标本严重 溶血时，两种方法的测定结果均偏高。 ,关键词, 电化学发光 免疫分析法;放射免疫分析法;血清铁蛋白 To Assess the Determination of Serum Ferritin In ECLIA and RIA Abstract: Objective To explore the significance of the determination of Ferritin in ECLIA and RIA.Methods Using the ECLIA and RIA to do experiment on the sample SF of the middle concentration with the withdraw and antiinterference test respectively(the interference is Hb),and to repeat doing the experiment on the sample SF of the lower or the higher concentration.Results The results showed that the mean withdrawing rate of ECLIA was 95.6% and that of RIA is 93.5% ,which showed no differences when compared (P0.05).The CV of ECLIA is 3.0% 、4.3% and that of RIA is 8.5%、9.3% ,which showed the certain differences when compared (P0.05).The Hb level is between 1.0 to 6.0 g/L ,as the concentration of Hb increases,the interference ability is also increased.The CB of the determinated result is more than 5.0% when using ECLIA to determinated SF as the concentration of Hb?4.0% and using RIA to determinaed SF as the concentration of Hb?2.0%.Conclusion The two methods of the determination of SF in ECLIA and RIA are precise and accurate,but ECLIA is better than RIA.The determination results are all higher when serious hemolysis occurred. Key words:ECLIA;RIA;Ferritin 铁蛋 白是人体内一种含铁丰富的蛋白，主要分布于肝、脾、骨髓等组织细 胞中。正常情况下，体内血清铁蛋白(SF)含量甚微，它是诊断缺铁性 贫血、铁负荷过度等疾病的有效指标,1,。此外，许多实体恶性肿瘤 细胞可以合成和分泌铁蛋白，故可作为肿瘤标志物，用于肿瘤诊断 ,1,。SF测定值的增高或降低，均有重要临床意义，因此必须准确 测定。本文对电化学发光免疫分析法(ECLIA)