分子标记辅助选择定向改良不育系Q211S稻瘟病的抗性.PDFVIP

中国农学通报 2018,34(24): 135-140 Chinese Agricultural Science Bulletin 分子标记辅助选择定向改良不育系Q211S稻瘟病的抗性 黄艳玲，严 志，王 慧，申广勒，张从合 （安徽荃银高科种业股份有限公司/农业部杂交水稻新品种创制重点实验室，合肥230088 ） 摘 要：为提高水稻温敏型核不育系‘Q211S ’的稻瘟病抗性，以‘Q211S ’为受体材料，以携带Pi9 基因的 抗稻瘟病材料‘75- 1- 127’为供体，通过常规育种方法和分子标记辅助选择技术相结合，选育出5 个抗病 性改良的两系不育系。抗性鉴定及田间试验结果表明：这5 个抗病性改良的两系不育系对苗瘟、穗瘟均 表现较高抗性，其他特征特性与‘Q211S ’相近。5 个改良不育系与‘Q211S ’所配组合在株高、每穗总粒 数、结实率、千粒重上差异不显著；而改良不育系所配组合在稻瘟病抗性方面较‘Q211S ’所配组合明显 增强。 关键词：水稻；分子标记辅助选择；稻瘟病；Pi9 基因；不育系；Q211S 中图分类号：S511 文献标志码：A 论文编号：cas DirectedImprovementofRiceBlastResistanceofSterileLineQ211Swith MolecularMarker-AssistedSelection HuangYanling,YanZhi,WangHui,ShenGuangle,ZhangConghe (AnhuiWin-AllHi-TechSeedCo.,Ltd/KeyLabforNewVarietyDevelopmentof HybridRiceofMinistryofAgriculture,Hefei230088) Abstract: Toimprovethericeblastresistanceofthermo-sensitivegenicmalesterileline ‘Q211S’,the thermo-sensitivegenicmalesterileline ‘Q211S’wasusedasthereceptorparentandthe‘75-1-127’with excellentriceblastresistance,carryinggenePi9,wasusedasdonorparent,fiveimprovedtwo-linemalesterile lineswith riceblastresistancewerebredbyusingconventionalbreedingandmolecularmarker-assisted selection.Resistanceidentificationandfieldtrialsdemonstratedthatthefiveimprovedtwo-linemalesterile linesperformedhigherresistancetoriceleafblastandpanicleneckblast,theotheragronomiccharacterswere almostidenticalto thatof ‘Q211S’.Furthermore,thenewhybridricecombinationswhichthefiveimproved two-linemalesterilelinericewereusedasfemaleparentshadnosignificantdifferencesinplantheight, spikeletnumberperpanicle,settingrateand 1000-grainweightcomparedwiththatof ‘Q211S’usedas femaleparent,whereasthericeblastresistancesofthenewhybridricecombinationswhichthe