植物生长素对大豆下胚轴不定根诱发过程中组织内过氧化酶基因之影响.DOCVIP

植物生長素對大豆下胚軸不定根誘發過程中 組織內過氧化酶基因之影響 Effect of auxins on peroxidase gene in hypocotyl cuttings of soybean during adventitious root formation 研究生：周建宏 Chou, Chien-Hung 指導教授：劉景煌 Liu, Zin-Huang 江友中 Chiang, Yu-Chung 【摘要】 植物生長素可以促進大豆下胚軸誘發不定根，使用不同的植物生長素會有不同的發根情形。Indole-3-butyric acid (IBA)在促進大豆下胚軸誘發不定根時，不定根會佈滿整株下胚軸。而相同濃度的Indole-3-acetic acid (IAA)和naphthaleneacetic acid (NAA)所誘發的不定根會集中在下胚軸的底部。將扦插兩天的大豆下胚軸進行分析，處理組的陰性 (pI 3.7) 和陽性 (pI 8.5) 過氧化酶活性都比控制組低；IBA處理組的內生性IAA濃度是控制組的1.5倍。利用genome walking的方法，進行 pI 8.5 POX 基因啟動子的選殖，並利用軟體分析可能的cis-elements，發現了兩個auxin response elements。由上述的結果可以推測，不定根發育的起始期(initiation phase)，外加的植物生長素會影響POX基因的表現，使內生性IAA含量增加，促使細胞分裂和延長。 關鍵字：不定根、大豆、過氧化酶、吲哚乙酸、吲哚丁酸、萘乙酸 【Abstract】 The auxins promoted rooting in soybean (Glycine max L.) hypocotyls. Different auxins induced adventitious roots dissimilar development. Indole-3-butyric acid (IBA) treatment increases root number dramatically while simultaneously stimulating root formation along the whole soybean hypocotyl. Indole-3-acetic acid (IAA) and naphthaleneacetic acid (NAA) at the same concentration even reduce root number, and the roots formed most appear in the lowermost part of the cutting base. At the end of the treatment, on the day 2, the levels of IAA increased dramatically in the IBA-treated tissues. The content of IAA in the IBA-treated tissues is about 1.5-fold higher than that of the control tissues. The activity of anionic peroxidases (POX, pI 3.7) and cationic POX (pI 8.5) was significantly inhibited by exogenous auxins during the induction of adventitious roots on the day 2 and day 3. We focus on a cationic POX (pI 8.5) that was used PCR-based genome walking method to find its promoter sequence and analysis its cis-elements. The pI 8.5 POX gene promoter contains two sites that are homologous to sequences commonly found in auxin response elements. Once POX gene expression is suppressed by auxins before adventitious rooting, the endogenous IAA may increase, which would result in the increased level o