基于超氧化物歧化酶的生物传感器的研究-应用化学专业论文.docxVIP

筚干超氧化物歧化酶的生物传感器的研究 筚干超氧化物歧化酶的生物传感器的研究 摘嘤 摘 要 本文结合自组装法、纳米技术和溶胶-凝胶法，制备了自组装溶胶一凝胶酶电极 (SOD／Cys／sol-gel)、金溶胶自组装酶电极(SOD／Cys／AuNP)和金溶胶自组装溶胶． 凝胶酶电极(SOD／Cys／AuNP／sol-gel)。系统地研究了pH和扫速对三种新颖的酶电 极的峰电流和峰电位的影响，优化了酶电极的制备条件，并考察了酶电极的稳定性。 以二甲亚磺l／氢氧化钠和黄嘌呤／黄嘌呤氧化酶体系产生超氧阴离子，研究了酶电极 埘其响应性能。实验表明：加入L-半胱氨酸，有利于SOD在电极表面膜中的取向， 从而大大增加了酶电极的灵敏度。加入金溶胶，催化效果明显，进一步提高了酶电 极的灵敏度。溶胶．凝胶不仅能有效地固定酶，而且能很好地保持其活性。 SOD／Cys／AuNP／sol—gel酶电极阴阳极峰电流之比Wk=1．1，阴阳极峰电位的差值 △ED=O．24 V(100 mV s1)，式量电位为110 mV(参比电极为饱和甘汞电极)，呈现 准町逆的电化学过程。估算得到其电子转移速率常数为10．3 s-1。放置60天，活性 保持97％。实验证明：综合自组装法、纳米技术和溶胶一凝胶法在制备生物传感器方 面的诸多优点，制备的SOD／Cys／AuNP／sol—gel酶电极不仅灵敏度高，而且稳定性好。 关键词：超氧化物歧化酶，超氧阴离子，金溶胶，L一半胱氨酸，自组装技术，溶胶． 凝胶法。 作 者：彭少华 指导老师：狄俊伟 AbstractThree Abstract Three kinds of novel superoxide dismutase(SOD)electrodes(SOD／Cys／sol-gel， SOD／Cys／AuNP and SOD／Cys]AuNP／sol—gel)were developed by combining self-assembled monolayers(SAMs)，nanotechnology and sol—gel technology．The effect of pH and scan rate on the peak current and peak potential of SOD electrodes and their stability were studied systematically．The preparation conditions were optimized The SOD electrodes were used to respond to snperoxide anion，which was prepared by DMSO／NaOH and xanthine／xanthine oxidnse svstems．The faster electron transfer rate of SOD electrodes witIl higher sensibility and excellent stability in the presence of L-cysteine and catalysis of Au nanoparticles was testified．The sol—gel film call not only immobilize SOD efficiently,but also maintain its reactivity well．The current ratio of the cathodic peak current to the anodic one of the SOD／Cys／AuNP／sol—gel electrode (ipc／ipa=1．1 at 100mV sq)and the separation between the anodic and cathodic peak potentials(△Ep_Epa—EPc-O．24 V at 100mVs“)indicate that the electrode process of SOD is quasi—reversible．The formal potential，estimated as(E矿Epc)／2，was 1 I OmV(versus SeE)．The heterogeneous electron transfer rate constant was evahmted to be about 1 0．3 s一．The reactivity of electrode，which was stored after sixty days，maintained 97％ Making use