铁棍山药PPO的最适底物热稳定性及褐变控制研究-河南农业科学.PDF

DOI :10.15933/j.cnki .1004 -3268.2010.12.001 河南农业科学 PPO 、 1 1,2 ＊ 1 1 1 1 1 1 刘　莹, 赵喜亭 , 赵月丽,鲁梦娇 , 成淑君 ,赵雪蕾 ,李明军 ,刘亚丽 (1. , 453007; 2., 453007) :研究了铁棍山药多酚氧化酶(PPO )的最适底物、热稳定性及不同抑制剂对其活性的影 。 结果表明:在试验范围内,铁棍山药PPO 最适底物为邻苯二酚;在较低温度(20 ～40 ℃)下其PPO 能保持较稳定的活性,较高温度(40 ～80 ℃)下其活性快速下降,80 ℃下保温10min, PPO 活性几乎 为零;抑制剂对PPO 活性的抑制效果依次为:L-抗坏血酸L-半胱氨酸盐酸盐植酸柠檬酸。 :铁棍山药;多酚氧化酶;热稳定性;褐变 :TS255.3　　:A 　　:1004-3268(2010)12-0095-04 Studies on the Best Substrate, the T hermal Stabilit of Pol phenol Oxidase and the Brow ning Control in D ioscorea op p os tia T hunb.cv .T iegun 1 1,2 ＊ 1 1 1 LIU Ying ,ZHAO Xi-ting ,ZHAO Yue-li , LU M eng-jiao ,CHENG Shu-jun , 1 1 1 ZHAO Xue-lei , LI Ming-jun , LIU Ya-li (1.College of Life Science, Henan Normal Universit ,Xinxiang 453007, China; 2.Engineering Technolog Research Center of Nursing and Utilization of Genuine Chinese C rude Drugs of Henan Province Universit ,Xinxiang 453007, China) Abstract:The best substrate, the thermal stabilit of pol phenol oxidase (PPO )from D ioscorea opp ost ia Thunb .cv.Tiegun and the effects of inhibitor on its activit w ere studied in this paper. The results show ed that :The best substrate of PPO from D ioscor ea op p osti a Thunb.cv .Tiegun w as catechol in the range of this experiment;the activit of PPO w as more stable under the low er temperature (20-40 ℃),w hile, its avtivit declined rapidl under the higher temperature(40- 80 ℃)and w as inhibited almost completel w hen incubated for 10 min under 80 ℃;the order of in- hibition effect from stro