碱性脂肪酶的分析-生物学专业论文.docxVIP

摘要逶过Rhodamine 摘要 逶过Rhodamine B平擞簿逡，鬏捺炎毙瓣太小薅逡褥戮二拣产l誓麓薅能力较 强憨蘩秘：缨蘩,41caligenes sp翻霉蘩Penicillium expansum。 在摇瓶条伴下对Alcaligenes sp鹣产酶≮艺参数遴行了研究，包括镟源和氮 源的种类和浓度、营养挫、培养湿度、初媲pH僵、袭嚣溪性刘等。在越基皴上 浆用优化殿的培莠基和焙葬条镣，农3L发黪罐孛进行产酶试验，绫聚袈鸦；遴 气爨和搅拌速度愚影噙Alcaligenes sp会成l翼膀酶购熬要趿素，其潺宣发酵条昝 分剐怒O．5vvm、400rpm，发酵液中碱性脂肪酶活力达到225IU／ml。 程摇瓶条佟下对Penicittium expansum产酶培养熬和产酶条俘避李亍丁优化， 瓴撼缓源、氮源、培养滠魔、禳始p薹重德、表碉活瞧剂、摇瓶装液璧和转速等。 采用谯纯辫静培养蓬及绩箨条件，Penicillium expansum的碱径脂精酶活力可遮 700IU／ml。3L发酵罐中的放大产酶试验袭明，溶氧对Peniciltium expansum会成 g肇髓麓至关重烫。 ，瓣鼹摹孛l鬟黪懿瓣酶学熬矮滋程了磺究：Alcaligenes sp鼗茨懿在p珏8．o～l蛰．0 范围内稳定，Penicillium expansum脂肪酶在pH 7．o～9．5藏围内稳定；逝种脂肪 酶最遥作麓龋谴均麓9．0～9．5，均满予碱性脂肪酶，可用季棼洗涤荆用酶。 Alcaligenes sp精貉酶程55℃戳下较为稳宠，最适菠鹰溢淡为40．c；Penicillium expansum§瓣赫鹬淼弱℃骧下较为稳定，最邋反应激发为30～35℃。Ca2+，M甓2+ 阍为蹰种酶的促进剂；Cu”和Fe2+因为两耱酶蛇郡测粼；Na+怒Penicillium expansum滕脐海的促进劐，Alcatigenes sp月鬟髓酶鹣毒露粼剩。 微生甥碱靛镕器骆酶舆骞霪簧数经济铃篷，嚣兹瓣遮类酶潮裁熬徽产溪凌澎耩 空白。本论文的研究结果在学术上和应用中都具有蘑要意义。l∥～—一 关键调：菌种筛选}碱性月鬟腊瓣}发赘} 酶学性震} ABSTRAC譬By ABSTRAC譬 By screening with Rhodamine B plate method，two strains which had high ability to produce lipase were obtained．One iS bacteria called Alcaligenes sp，the other is epiphyte named PeniciUium ex_pansum． The fermentation parameters of Alcaligenes sp were studied in shaking flask， including carbon sources，nitrogen sources，nutrition salts，cultivate temperature, initial pH value and surface active agent，etc．The fermentation results in 3L fermentor indicated that aeration and stirring speed had obviously influence the synthesis of lipase by Alcaligenes sp．The optimal aeration and stirring speed were o．5vvm and 400rpm respectively,wi攮which the alkaline liPase activity could reach 225IU／m1． 秘le compositions of medium and culture conditions for Penicillium expansum were also studied in shaking flasks，the alkaline lipase activity was 700 IU／ml，under the optimal medium and culture conditions．The results in 3L fermentor showed the 02 in the medium was fairly important for Peniciltium expansum synthesize lipase． Alcaligenes sp lipase and Penicillium expansum lipase were stabl