秸秆腐熟菌剂的细菌种群分析及其腐熟过程的动态研究-微生物学专业论文.docxVIP

Abstr·actIn Abstr·act In this study,the bacteria composition and the dynamic changes of the sUaw-decomposing inoculants were analyzed by culture dependent method and molecular biology method．The 16S rDNA clone library method is more impersonal and exactly for analyzing the samples’bacteria composition than the culture dependent method．Now,PCR-DGGE is one of the best methods to龇udy the dynamic changes of inoculants in the process of fermentation．The result of this snldy would offer help for the rational composition、production and application of straw-deonmposing inoenlants．The main results of this study were鹋foUows． Using 16S rDNA clone library method，the bacteria composition of the sample A and B were investigated．The results indicated tll at：the dominant communities of sample A were Weissella confuse． Bacillus subalis and Bacillus pumilua，and the dominant communities of sample B were Lactobacillua buchnerl,Lactobacillusfarciminis and Lactobacillus acetotoleran$．This s“ldy showed that 16S rDNA clone library method had dicer application perspectives in analying the bacteria of microbial inoenlants and its quality control than the culture dependent method． Using DGGE，two forms of fermentions were analyzed，one was aerobic，the one was anaerobe．Their image patterns had much difference from each other,but the two samples’image patterns of the same form of fermention w雠similar．And the interesting bends’sequencing results wore different from sample’S bacteria composition． The sm棚compostjoined sample A and B Was analyzed with DGGE and culture dependent method． mainly studying the dynamic changes of microbe．At the saI∞time．we also mensuratcd the interrelated parametex,such as temperature，pH，water content，C／N and seed germination rate．The msults showed that．the of sUaw抵mposing inoculants could make the compost go on wheels；the microbe in compost had obviously succession．First,the lactobacillus and bacteria of normal tempemre increased strongly,then the temperature was heighten