接种费氏中华根瘤菌WGF03及其硫代谢突变体对大豆生长和根际细菌群落的影响-生物化学与分子生物学专业论文.docxVIP

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接种费氏中华根瘤菌WGF03及其硫代谢突变体对大豆生长和根际细菌群落的影响-生物化学与分子生物学专业论文.docx

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II II 型 WGF03 还是突变体 105.26 和 CysH2+17 根瘤菌对大豆根际细菌群落物种多样性和群落结构均有一定影响，主要体现在影响根际部分细菌类群和细菌生物量。大豆生长发育阶段是影响大豆根际细菌群落的主要因素。对 DGGE 图谱优势条带测序结果表明，优势菌的种属繁多，不存在特定的优势种属菌； Proteobacteria 为大豆根际土壤的绝对优势类群，其中以 α-Proteobacteria、γ-Proteobacteria 为主。综上所述，野生型 WGF03 能够成功侵染大豆根系从而发挥了促进大豆生长并增加产量的作用，其两株突变体因硫酸盐代谢功能不完全而没有发挥作用；三株根瘤菌菌株对大豆根际细菌群落有一定的影响，此影响可能通过与大豆互作而间接产生，此结论和其中机制需要重复实验和进一步探索证明。关键词费氏中华根瘤菌 DGGE 根际细菌 PAGE PAGE IV EFFECTS OF SINORHIZOBIUM FREDII WGE03 AND ITS SULFUR METABOLIC MUTANTS INOCULATION ON SOYBEAN GROWTH AND RHIZOSPHERE BACTERIAL COMMUNITIES ABSTRACT It was the first time to study the effects of Sinorhizobium fredii WGF03 and its two mutants inoculation on soybean growth and rhizosphere bacterial communities in the field condition. The mutants were 105.26 and CysH2+17, whose sulfur metabolic relevant gene cysDN or cysH was destroyed respectively. There were four treatments for soybean planting. They were WGF03 inoculation, 105.26 inoculation, CysH2+17 inoculation and non-inoculation. The plant height, dry weight, product and nodule number were used to analyze the inoculation impacts on soybean growth. SPSS analysis of the plant height and dry weight showed that the treatment of WGF03 inoculation was significantly better than other treatments. Meanwhile, there was no significant difference among the treatments of 105.26 inoculation, CysH2+17 inoculation and non-inoculation. What’s more, compared to the treatment of non-inoculation, average nodule numbers of flower and mature stages showed that the treatment of WGF03 inoculation was 24.08 % and 22.99 % higher, and average yield increased 39.08 %. Those measurements of the other three treatments stayed the same. The rhizosphere soil of all treatments were carefully collected on the stage of flower and mature. And then 16S rDNA V3 region and V6-V8 region were amplified after extracting the total microbial DNA of each soil sample, finally the DGGE gel was run. Bio-softs such as Quantity One V4.6