金雀异黄酮对去卵巢大鼠血清SOD和MDA以及脑突触体的影响-人体解剖学与组织胚胎学专业论文.docxVIP

朱建华硕士如文 朱建华硕士如文 中文摘要 摘除双侧卵巢。术后第2天开始皮下注射给药，隔日1次；术后8周检测各组动 物血清SOD及MDA值、大脑皮质和海马脑突触体[Ca2+1i i：突触体膜相对粘滞 度，以及海马突触体素免疫阳性产物平均光密度变化。 结果：大鼠血清SOD及MDA值在假手术组、金雀异黄酮组、苯甲酸雌二醇 组之间无显著性差异◇O．05)，但OVX组SOD值显著低于其它3组t萨O．05)， MDA值显著高于其它3组(F0．05)；去卵巢对照组大脑皮质和海马突触体[Ca2+]i 均显著高于其它组(球O．01)，但在假手术组、苯甲酸雌二醇组及金雀异黄酮组之 间不存在显著性差异(pO．05)；OVX组海马突触体膜相对粘滞度显著高于假手 术组、苯甲酸雌二醇组p0．05)和金雀异黄酮组0O．01)，但假手术组、苯甲 酸雌二醇组和金雀异黄酮组之间不存在显著性差异(矿O．05)；OVX组CAl、CA2、 CA，区放射层和齿状回分子层的免疫阳性产物平均光密度值均显著低于假手术 组、金雀异黄酮组和苯甲酸雌二醇组(PO．叭)，但后3组之间无显著性差异 仞O．05)。 结论：金雀异黄酮具有类似于雌二醇的抗氧化作用，能增加去卵巢大鼠SOD 含量，降低氧自由基水平，减轻脂质过氧化反应；从而阻断由氧化应激、脂质过 氧化等引起的钙超载，切断氧化应激的恶性循环，恢复去卵巢大鼠海马神经元之 间的突触联系。金雀异黄酮有望作为一种雌激素替代药用于女性更年期后中枢神 经系统退行性疾病的防治。 关键词：金雀异黄酮；雌激素替代疗法；氧化应激；钙超载；突触体 II 叟 叟 朱建华硕士论文 英文摘要 Effects of Genistein 011 Serum SOD，MDA and Cerebral Synaptosome of Ovariectomized Rats Major AnatomyHistology and Embryology Master candidate Zhu Jianhua Supervisor Xu Jie Associate Professor Abstract Baekground：Alzheimer’S disease(AD)is a neurodegenemtive disorder characterized by pmgressive impairment of cognition，senile plaque，neurofibrillary tangles，cholinergic neurons loss in basal forebrain and synapse loss．Epidemiological study shows that the incidence of female AD increases significantly after menopause， which accounts to two to three times aS that of male．Estrogen replacement therapy (ERT)that could decrease the incidence or postpone the onset ofAD and increase the survival numbers and functions of memory-related neurons in brain Was an important method to prevent and cure AD of postmenopausal female．But ERT is restricted by some side effects，such as increased incidence of mammary cancer and carcinoma of endometrium．Genistein is a phytoestrogen，which affmity tO estrogen receptor alpha is about one twenty-sixth as that of 1 7一beta estradiol，and to estrogen receptor beta is equal to that of 1 7一beta estradi01．It has the ability to penetrate the blood．brain barrier easily，but has no side effects as estradi01．Therefore，it has the potential to become a promising substitute ofestra