金(I)配合物的合成、表征及生物活性研究-生物化学与分子生物学专业论文.docxVIP

摘要 摘要 I I 要 金(I)配合物具有多变的几何构型和氧化还原态，呈现独特的热力学和动力学 特性，并在催化、荧光、蛋白抑制剂、抗肿瘤等领域备受关注。本论文合成卤素 系列金(I)配合物和含硫系列金(I)配合物两大类，并对其生物活性进行研究，总共 分为五章： 第一章，介绍了论文的研究背景，总结概述金(I)配合物的研究现状，特别是 金(I)配合物的荧光、催化性质、抗肿瘤活性等方面。 第二章，合成卤素系列金(I)配合物，采用核磁、红外进行结构表征，并解析 出三苯基膦氯金的晶体结构。 第三章，合成系列硫脲衍生物，并以硫脲衍生物或巯基衍生物作为配体，进 一步反应得到含硫系列金(I)配合物，采用核磁、红外进行结构表征。其他含硫金 配合物的合成反应条件仍在优化。 第四章，研究上述金(I)配合物对脲酶的抑制活性、对血清白蛋白（BSA，HSA） 的荧光猝灭作用和抗菌活性。结果表明：(1) 化合物 2 的脲酶抑制活性最好，MIC50 值为 0.57μg/ml；(2) 化合物 1-4 对血清白蛋白的猝灭方式都为静态猝灭；(3) 化合 物 1 和 2 的抗菌活性最好，其中化合物 1 抑制大肠杆菌、化合物 2 抑制枯草芽孢 杆菌和金黄色葡萄球菌的 IC50 值均小于 0.05μg/ml。 第五章，对全文内容进行总结，并作出对今后工作的展望。 关键词：金(I)配合物，脲酶，血清白蛋白，荧光猝灭，抗菌 ABSTRACT ABSTRACT II II ABSTRACT Gold(I) complexes with various geometry configuration and redox state, which present unique thermodynamic and kinetic characteristics have been paid much more attention in catalysis, fluorescence, protein inhibitors, antitumor activities etc. In this paper, the halogen-containing gold(I) complexes and sulfur-containing gold(I) complexes have been designed and synthesized, and the biological activities of these compounds have also been investigated. This thesis is divided into five chapters. Chapter 1, the study background and status of gold(I) complexes were summarized, especially in fluorescence, catalysis and antitumor activities. Chapter 2, halogen-containing gold(I) complexes were synthesized and characterized by NMR and IR analysis. Compound (triphenylphosphine)gold(I) chloride has been structural characterized by X-ray single-crystal determination. Chapter 3, a series of thiourea derivatives and sulfur-containing gold(I) complexes with thiourea derivatives and mercapto derivatives were synthesized and characterized by NMR and IR analysis. The reaction conditions of other sulfur-containing gold(I) complexes still needs optimization. Chapter 4, the urease inhibitory activities, the fluorescence quenching of BSA or HSA activities, and antibacterial activities of gold(I) complexes have been investigated. The research results were as follows: (1)