新方法制备富血小板凝胶对兔颅骨骨缺损的成骨效果.pdfVIP

诱导新骨再生，完全修复骨缺损的作用，成骨效果远高于空白对照组，具 有统计学意义(P≤0.05)。统计学分析得到两种富血小板凝胶修复骨缺损的 作用无明显差异(P＞0.05)。 实验结论 新方法与添加牛凝血酶等激活剂均可完全修复骨缺损。虽然未发现牛 凝血酶对兔机体造成凝血紊乱，但随着时间的变化略有改变，因此尚不能 定论牛凝血酶是否能造成凝血紊乱。 关键词：富血小板凝胶 牛凝血酶 骨缺损 引导骨组织再生 凝血检测 5 New Method For Preparing The Osteogenic Effect Of Platelet-rich Gel On The Rabbit Skull Bone Defects Abstract Experimental Purpose Through animal experiments, the effect and feature of the platelet-rich gel without activators guiding the bone regeneration in the repair of bone defect can be evaluated. In order to provide a reference for clinic, Comparing with the effect of the platelet-rich gel with activators in osteogenesis effects, and detecting whether the activator caused coagulant disorders is necessary. Experimental Methods 36 rabbits for research are divided into three groups, the new method group, the platelet-rich gel with activators such as bovine thrombin group, and control group. Examining the hematoxylin-eosin staining (HE staining) and Masson trichrome staining of the bone tissue slice by X-ray. Analyze the status of bone defects in 2 weeks, 4 weeks, 8 weeks, and 12 weeks, and examine the two research groups. Experimental Result The research is successful. After surgery, the animal for research has no complication, for example, wound infection, and dehiscence. The X-ray image of the bone tissue shows the all of the methods have osteogenesis effects. The periosteum is higher than control group (P≤0.05). The sclerous tissues slice of bone samples show all of the two methods can make the bone regeneration, and repair the bone defects completely. There has no obvious difference about the two platelet-rich gels in osteogenesis effects by statistical analysis. Experimental Conclusions 6 A reasonable animal model is established in this research