结直肠癌外周血循环肿瘤细胞检测的临床诊疗价值研究-普外科专业论文.docxVIP

3 3 目 录 英文缩略词表······················································1 中文摘要···························································2 英文摘要···························································4 引言·······························································6 1 材料与方法··················································8 2 结 果······················································11 3 讨 论······················································14 4 结 论······················································23 参考文献··························································24 附 件·····························································31 致 谢·····························································33 综 述·····························································34 福建医科大学硕士学位论文 福建医科大学硕士学位论文 PAGE PAGE 10 英文缩略语表 (Abbreviation) 缩写 英文全称 中文全称 CTC circulating tumor cell 循环肿瘤细胞 PBMC Peripheral blood mononuclear ce11 外周血单核细胞 Ep-CAM epithelial cellular adhesion molecule 上皮细胞粘附分子 CK19 Cytokeratin 19 细胞角蛋白19 FCM Flow Cytometry 流式细胞技术 EGFR endothelial growth factor receptor 内皮生长因子受体 PCR Polymerase Chain Reaction 聚合酶链式反应 RT-PCR Reverse transcription-PCR 逆转录聚合酶链反应 AJCC American Joint Committee on Cancer 美国癌症联合委员会 PFS Progress Free Survival 无进展生存期 OS Overall Survival 总生存期 PBS phosphate buffer solution 磷酸缓冲液 FITC fluorescein isothiocynate 异硫氰酸荧光素 PE phycoerythrin 藻红蛋白 PerCP peridinin chlorophyll protei 多甲藻黄素叶绿素蛋白 EMT Epithelial-Mesenchymal Transition 上皮间质化 【目的】 结直肠癌患者外周血循环肿瘤细胞检测的临床诊疗价值研究 摘 要 本实验应用密度梯度离心法联合流式细胞技术检测外周血中结直肠癌循环肿瘤 细胞，分析其与浸润深度、淋巴结转移、TNM 分期、肿瘤分化程度等关系，探讨运用 流式细胞仪检测结直肠癌患者外周血循环肿瘤细胞（circulating tumor cell，CTC） 的临床价值。 【方法】 1 采集 2014 年 5 月 1 日至 2015 年 1 月 31 日莆田学院附属医院胃肠外科 60 例结 直肠癌患者外周血 10ml，采集 2014 年 6 月 1 日至 2014 年 11 月 31 日共 15 例健康 志愿者外周血 10ml 作为对照组。 2 外周血淋巴细胞分离法制备外周血单个核细胞悬液，平分为试验管和对照管，试 验管分别标记 CK19、EP-CAM、CD45 单克隆抗体。阴性对照管分别加入同型对照抗体。 3 标记好的外周血单个核细胞通过流式细胞仪，检测 CK19+/ EP-CAM +/CD45-的细胞， 作为结直肠癌患者 CTC 的标志。 4 所得结果用 SPSS 13.0 统计软件包处理，实验结果应用χ2 检验统计分析，以 P ＜0.05 为差异有统计学意义。 【结果】 1